Development of the methods for detection of diatoms utilizing silica binding abilities of DNA and proteins

• Project/Area Number: 26460882
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Oita University
• Principal Investigator: Seo Yasuhisa 大分大学, 医学部, 助教 (80187830)
• Co-Investigator(Kenkyū-buntansha): 岸田 哲子 大分大学, 医学部, 教授 (50136793) ; 内田 智久 大分大学, 医学部, 助教 (70381035)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 溺死 / プランクトン / 珪藻 / 酵素染色 / ストレプトアビジン / プランクトン検査 / スリレラ属 / 染色

Outline of Final Research Achievements

It was confirmed that some kinds of peroxidase-labeled streptavidin and IgG has abilities of non-specific adsorption to glassy matter. The methods of staining for diatom frustules were established. Then this staining method was applied to diatom tests that have been actually drowned victims. As a result, detection of the diatom frustules was easier, and the time to diagnose drowning was greatly shortened.
In addition, we could submit a paper on novel species of diatom discovered from sample water obtained from a drowning site during this research. Subsequent research confirmed that this diatom was also found in a completely different water system in Oita prefecture, and expansion of the habitat was found. Currently, we are conducting comparative study on the genetic diversity of diatoms discovered from each water system.

Research Products

• [Presentation] 大分川で発見された新種珪藻の分子系統学的解析 (2017)
  • Author(s): 山口翔、岸田哲子、瀬尾泰久、黒木浩二
  • Organizer: 日本法医学会九州地方会 （那覇市、サザンプラザ海邦）
• [Presentation] 壊機法における珪藻検出率低下の原因探求 (2016)
  • Author(s): 長野友樹、岸田哲子、瀬尾泰久、黒木浩二
  • Organizer: 日本法医学会九州地方会
  • Place of Presentation: 福岡県 久留米市 久留米大学筑水会館
  • Year and Date: 2016-10-14
• [Presentation] ある溺死体揚収現場から発見された新型珪藻について (2015)
  • Author(s): 宗像剛史、岸田哲子、瀬尾泰久、黒木浩二、細井利男、真山茂樹
  • Organizer: 日本法医学会九州地方会
  • Place of Presentation: 宮崎県宮崎市 宮崎県医師会館
  • Year and Date: 2015-10-16
• [Presentation] 珪藻の染色法-第2報- (2014)
  • Author(s): 青木泰佑、岸田哲子、瀬尾泰久、黒木浩二
  • Organizer: 日本法医学会九州地方会
  • Place of Presentation: 鹿児島県市町村自治会館（鹿児島）
  • Year and Date: 2014-10-10 – 2014-10-11