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Development of the methods for detection of diatoms utilizing silica binding abilities of DNA and proteins

Research Project

Project/Area Number 26460882
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionOita University

Principal Investigator

Seo Yasuhisa  大分大学, 医学部, 助教 (80187830)

Co-Investigator(Kenkyū-buntansha) 岸田 哲子  大分大学, 医学部, 教授 (50136793)
内田 智久  大分大学, 医学部, 助教 (70381035)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords溺死 / プランクトン / 珪藻 / 酵素染色 / ストレプトアビジン / プランクトン検査 / スリレラ属 / 染色
Outline of Final Research Achievements

It was confirmed that some kinds of peroxidase-labeled streptavidin and IgG has abilities of non-specific adsorption to glassy matter. The methods of staining for diatom frustules were established. Then this staining method was applied to diatom tests that have been actually drowned victims. As a result, detection of the diatom frustules was easier, and the time to diagnose drowning was greatly shortened.
In addition, we could submit a paper on novel species of diatom discovered from sample water obtained from a drowning site during this research. Subsequent research confirmed that this diatom was also found in a completely different water system in Oita prefecture, and expansion of the habitat was found. Currently, we are conducting comparative study on the genetic diversity of diatoms discovered from each water system.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2017 2016 2015 2014

All Presentation (4 results)

  • [Presentation] 大分川で発見された新種珪藻の分子系統学的解析2017

    • Author(s)
      山口翔、岸田哲子、瀬尾泰久、黒木浩二
    • Organizer
      日本法医学会九州地方会 (那覇市、サザンプラザ海邦)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 壊機法における珪藻検出率低下の原因探求2016

    • Author(s)
      長野友樹、岸田哲子、瀬尾泰久、黒木浩二
    • Organizer
      日本法医学会九州地方会
    • Place of Presentation
      福岡県 久留米市 久留米大学筑水会館
    • Year and Date
      2016-10-14
    • Related Report
      2016 Research-status Report
  • [Presentation] ある溺死体揚収現場から発見された新型珪藻について2015

    • Author(s)
      宗像剛史、岸田哲子、瀬尾泰久、黒木浩二、細井利男、真山茂樹
    • Organizer
      日本法医学会九州地方会
    • Place of Presentation
      宮崎県宮崎市 宮崎県医師会館
    • Year and Date
      2015-10-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 珪藻の染色法-第2報-2014

    • Author(s)
      青木泰佑、岸田哲子、瀬尾泰久、黒木浩二
    • Organizer
      日本法医学会九州地方会
    • Place of Presentation
      鹿児島県市町村自治会館(鹿児島)
    • Year and Date
      2014-10-10 – 2014-10-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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