Identification of Warburg effect inducer in pancreatic cancer

• Project/Area Number: 26461029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: Hamada Shin 東北大学, 医学系研究科, 助教 (20451560)
• Co-Investigator(Kenkyū-buntansha): 正宗 淳 東北大学, 医学系研究科, 准教授 (90312579)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵癌 / 細胞内代謝 / PSC-CM / miR-197 / ミトコンドリア

Outline of Final Research Achievements

This research project aimed to clarify the role of Warburg effect on malignant behavior of pancreatic cancer cells. For this purpose, we established tetracycline-inducible miR-197 expressing cell line. Induction of miR-197 suppressed oxygen consumption in this cell line. Furthermore, multiple components of oxidative phosphorylation and electron transport system were down-regulated. In addition, stimulation by pancreatic stellate cell-conditioned medium, was also found to affect cellular metabolism of pancreatic cancer.

Research Products

• [Journal Article] Exosomes Derived From Pancreatic Stellate Cells: MicroRNA Signature and Effects on Pancreatic Cancer Cells. (2017)
  • Author(s): Takikawa T, Masamune A, Yoshida N, Hamada S, Kogure T, Shimosegawa T.
  • Journal Title: Pancreas. Volume: 46 Issue: 1 Pages: 19-27
  • DOI: 10.1097/mpa.0000000000000722
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IL-6/STAT3 Plays a Regulatory Role in the Interaction Between Pancreatic Stellate Cells and Cancer Cells. (2016)
  • Author(s): Hamada S, Masamune A, Yoshida N, Takikawa T, Shimosegawa T.
  • Journal Title: Dig Dis Sci. Volume: Epub ahead of print
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Nrf2 knockout suppresses pancreatic carcinogenesis in a mouse model (2017)
  • Author(s): Shin Hamada, Atsushi Masamune, Keiko Taguchi, Masayuki Yamamoto, Tooru Shimosegawa
  • Organizer: Digestive Disease Week
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2017-05-21
  • Int'l Joint Research
• [Presentation] The MiR-365 Increases Gemcitabine Resistance by Regulating Cell Cycle and Apoptosis-related Molecules (2014)
  • Author(s): Shin Hamada, Atsushi Masamune and Tooru Shimosegawa
  • Organizer: 45th Anniversary Meeting of APA/JPS
  • Place of Presentation: Kohala coast, HI, USA
  • Year and Date: 2014-11-05 – 2014-11-08
• [Presentation] miR-197による癌細胞特異的代謝機構の制御 (2014)
  • Author(s): 濱田晋 正宗淳 下瀬川徹
  • Organizer: JDDW2014
  • Place of Presentation: 神戸 神戸国際会議場
  • Year and Date: 2014-10-23 – 2014-10-26