The establishment of novel endoscopic diagnostic method and biomarker for cholangiocarcinoma by fluorescence in situ hybridization

• Project/Area Number: 26461046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya City University
• Principal Investigator: NAITOH Itaru 名古屋市立大学, 大学院医学研究科, 講師 (30527750)
• Co-Investigator(Kenkyū-buntansha): 中沢 貴宏 名古屋市立大学, 大学院医学研究科, 研究員 (70305522) ; 宮部 勝之 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (00543985)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: FISH法 / 胆管癌 / IgG4関連硬化性胆管炎 / 胆管生検 / 染色体異常 / 7番染色体

Outline of Final Research Achievements

We evaluated the efficacy of fluorescence in situ hybridization (FISH) using transpapillary forceps biopsy specimens in the diagnosis of cholangiocarcinoma (CCA). We evaluated the copy number gains (> 2 copies) of CEP 3, 7, and 17 in patients with CCA and IgG4-related sclerosing cholangitis (IgG4-SC). The complementary use of FISH analysis using transpapillary forceps biopsy specimens improves the diagnostic yield for CCA in the differential diagnosis from IgG4-SC. Furthermore, a CEP 7 copy number gain may be a biomarker of poor prognosis in CCA patients.

Research Products

• [Journal Article] Autoimmune Hemolytic Anemia Obscured by the Obstructive Jaundice Associated with IgG4-related Sclerosing Cholangitis in a Patient with Type 1 Autoimmune Pancreatitis: A Case Report and Review of the Literature (2018)
  • Author(s): Yoshida M, Marumo Y, Naitoh I, Hayashi K, Miyabe K, Nishi Y, Fujita Y, Jinno N, Hori Y, Natsume M, Kato A, Iida S, Joh T.
  • Journal Title: Internal Medicine Volume: 57 Issue: 12 Pages: 1725-1732
  • DOI: 10.2169/internalmedicine.9818-17
  • NAID: 130007397141
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2018-06-15
  • Peer Reviewed / Open Access
• [Journal Article] Differential diagnosis of cholangiocarcinoma and IgG4-related sclerosing cholangitis by fluorescence in?situ hybridization using transpapillary forceps biopsy specimens (2018)
  • Author(s): Kato Akihisa、Naitoh Itaru、Miyabe Katsuyuki、Hayashi Kazuki、Kondo Hiromu、Yoshida Michihiro、Kato Hiroyuki、Kuno Toshiya、Takahashi Satoru、Joh Takashi
  • Journal Title: Journal of hepato-billiary pancreatic sciences Volume: 25 Issue: 3 Pages: 188-194
  • DOI: 10.1002/jhbp.536
  • Peer Reviewed / Open Access
• [Journal Article] Case of pancreatic metastasis from colon cancer in which cell block using the Trefle® endoscopic scraper enables differential diagnosis from pancreatic cancer. (2018)
  • Author(s): Kato A, Naitoh I, Kato H, Hayashi K, Miyabe K, Yoshida M, Hori Y, Natsume M, Jinno N, Yanagita T, Takiguchi S, Takahashi S, Joh T.
  • Journal Title: World J Gastrointest Oncol. Volume: 10 Issue: 3 Pages: 91-95
  • DOI: 10.4251/wjgo.v10.i3.91
  • Peer Reviewed / Open Access
• [Journal Article] IgG4-related Sclerosing Cholangitis with No Biliary Stricture but Severe Thickening of the Bile Duct Wall (2016)
  • Author(s): Shimizu S, Naitoh I, Nakazawa T, Hayashi K, Miyabe K, Kondo H, Nishi Y, Yoshida M, Umemura S, Hori Y, Kato A, Ohara H, Kuno T, Takahashi S, Joh T.
  • Journal Title: Internal Medicine Volume: 55 Issue: 12 Pages: 1575-1579
  • DOI: 10.2169/internalmedicine.55.6302
  • NAID: 130005157650
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] IgG4-Related Sclerosing Cholangitis. (2016)
  • Author(s): Nakazawa T, Shimizu S, Naitoh I.
  • Journal Title: Semin Liver Dis Volume: 36 Issue: 03 Pages: 216-228
  • DOI: 10.1055/s-0036-1584321
  • Peer Reviewed
• [Journal Article] Predictive factors for positive diagnosis of malignant biliary strictures by transpapillary brush cytology and forceps biopsy (2016)
  • Author(s): Naitoh I, Nakazawa T, Kato A, Hayashi K, Miyabe K, Shimizu S, Kondo H, Nishi Y, Yoshida M, Umemura S, Hori Y, Kuno T, Takahashi S, Ohara H, Joh T
  • Journal Title: J Dig Dis Volume: 17 Issue: 1 Pages: 44-51
  • DOI: 10.1111/1751-2980.12311
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Locus/Chromosome aberrations in intraductal papillary mucinous neoplasms analyzed by fluorescence in situ hybridization. (2015)
  • Author(s): Miyabe K, Hori Y, Nakazawa T, Hayashi K, Naitoh I, Shimizu S, Kondo H, Nishi Y, Yoshida M, Umemura S, Kato A, Ohara H, Joh T, Inagaki H.
  • Journal Title: Am J Surg Pathol. Volume: 39 Issue: 4 Pages: 512-20
  • DOI: 10.1097/pas.0000000000000360
  • Peer Reviewed / Open Access
• [Presentation] 胆管癌診断におけるFluorescence in situ hypridization(FISH)法を用いた新規胆管生検診断方法の有用性ーIgG4関連硬化性単価年との鑑別診断を中心にー (2017)
  • Author(s): 加藤晃久、内藤 格、林 香月
  • Organizer: 第94回消化器内視鏡学会総会
• [Presentation] 経乳頭的胆管生検検体を用いた胆管癌の診断能向上におけるFluorescence in situ hybridization(FISH)法の有用性 (2017)
  • Author(s): 加藤晃久，内藤格，林香月，坂哲臣，近藤啓, 吉田道弘, 藤田泰明, 夏目まこと, 城卓志
  • Organizer: 第93回日本消化器内視鏡学会総会
• [Presentation] Fluorescence in situ hybridization (FISH) 法を用いた経乳頭的胆管生検の胆管癌診断能 (2016)
  • Author(s): 加藤晃久，内藤格，林香月，坂哲臣，近藤啓, 西祐二, 梅村修一郎, 夏目まこと, 城卓志
  • Organizer: 第52回日本胆道学会学術集会
  • Place of Presentation: 新横浜プリンスホテル（横浜市）
• [Presentation] Predictive factors for positive diagnosis of malignant biliary strictures by transpapillary brush cytology and forceps biopsy (2015)
  • Author(s): Kondo H, Naitoh I, Nakazawa T, Hayashi K, Miyabe K, Shimizu S,Nishi Y, Yoshida M, Umemura S, Hori Y, Kato A, Ohara H, Joh T
  • Organizer: UEGW 2015
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2015-10-26
  • Int'l Joint Research
• [Presentation] Evaluation of Chromosomal Abnormalities by Fluorescence in Situ Hybridization in intraductal papillary mucinous neoplasm (2014)
  • Author(s): Miyabe K, Hori Y, Nakazawa T, Hayashi K, Naitoh I, Shimizu S, Kondo H, Nishi Y, Yoshida M, Umemura S, Kato A, Ohara H, Joh T, Inagaki H.
  • Organizer: DDW 2014
  • Place of Presentation: Chicago （United States）
  • Year and Date: 2014-05-04 – 2015-05-04
• [Book] 臨床消化器内科 (2018)
  • Author(s): 内藤 格、大原弘隆、中沢貴宏、清水周哉、林 香月、城 卓志
  • Total Pages: 8
  • Publisher: 日本メディカルセンター
  • ISBN: 4910094910283