| Project/Area Number |
26461105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
坂田 憲治 金沢大学, 附属病院, 講師 (20456411)
吉田 昌平 金沢大学, 附属病院, 助教 (30623657)
森 雅之 金沢大学, 附属病院, 医員 (30707526)
羽尾 裕之 兵庫医科大学, 医学部, 准教授 (40393243)
中西 千明 金沢大学, 附属病院, 助教 (80623660)
川尻 剛照 金沢大学, 医学系, 准教授 (90345637)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 血管内皮前駆細胞 / 冠動脈ステント / 新生内膜 / 血管内皮前駆細胞捕捉ステント / 内皮化 / 血管内皮細胞 |
|---|
| Outline of Final Research Achievements |
We developed VEGF-bound stents that may enable selective capture of EPCs followed by rapid endothelialization. Metallic stents were first coated with poly-(ethylene-co-vinyl alcohol), and then chemically bound with either VEGF. These stents were placed in porcine coronary arteries. Stent surface was evaluated by immunohistochemistry and by scanning electron microscope. VEGF-bound stents, small populations of KDR positive cells adhered to the stent struts. After long-term stenting, struts were fully covered with newly regenerated tissue. SEM images showed that the uniform tissue formed on struts was morphologically similar to native endothelium and was continuously connected with adjacent native endothelium. VEGF-bound stents provide highly selective capture of EPCs, followed by rapid formation of intact endothelium tissue at an early period of stenting. These results suggest that VEGF-bound stents could represent a promising therapeutic option for cardiovascular stenting.
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