Ischemic stroke therapy using quality and quantity-controlled culture system enriched in endothelial progenitor cells
| Project/Area Number |
26461320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永田 栄一郎 東海大学, 医学部, 教授 (00255457)
浅原 孝之 東海大学, 医学部, 教授 (20246200)
増田 治史 東海大学, 医学部, 准教授 (50278496)
木村 啓志 東海大学, 工学部, 准教授 (40533625)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 単核球培養 / 血管内皮前駆細胞 / 脳梗塞 / 細胞移植 / 単球培養細胞 / 単核球培養細胞 |
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| Outline of Final Research Achievements |
Quality and quantity-controlled culture system enriched in endothelial progenitor cells (EPCs) from unfractionated mononuclear cells (QQ-MNCs), which we developed, have a potential to activate anti-inflammatory cytokines and expanding vasculogenic EPCs. QQMNCs injected to mice at 1-day after occlusion of middle cerebral artery (MCA) reduced the rate of ischemic stroke volume compared with PBS. The immunohistochemistry showed the increase of positive cells reacted with VEGF and IL-10 after 7 and 21-days in QQ-MNCs-treated mice. These findings reveal that QQ-MNCs might promote repair and regeneration of neurovascular units after acute focal ischemic stroke.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Inositol Hexakisphosphate Kinase 2 Promotes Cell Death in Cells with Cytoplasmic TDP-43 Aggregation.2015
Author(s)
Nagata E, Nonaka T, Moriya Y, Fujii N, Okada Y, Tsukamoto H, Itoh J, Okada C, Satoh T, Arai T, Hasegawa M, Takizawa S.
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Journal Title
Mol Neurobiol.
Volume: -
Issue: 8
Pages: 5377-5383
DOI
Related Report
Peer Reviewed
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[Journal Article] Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.2014
Author(s)
H. Masuda, R. Tanaka, S. Fujimura, M. Ishikawa, H. Akimaru, T. Shizuno, A. Sato, Y. Okada, Y. Iida, J. Itoh, Y. Itoh, H. Kamiguchi, A. Kawamoto, T. Asahara.
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Journal Title
J Am Heart Assoc.
Volume: 3
Issue: 3
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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