AU-rich element dependent mRNA regulation in functional change of rheumatoid arthritis synoviocyte
| Project/Area Number |
26461463
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Kurume University (2016)
Hiroshima University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
杉山 英二 広島大学, 病院(医), 教授 (70179167)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | mRNA制御 / 3'UTR / AU-rich element / サイトカイン / 関節リウマチ / 滑膜細胞 / 発現制御 / mRNA / AU rich element / RNA結合タンパク / 3'非翻訳領域 / デュアルレポーター / アプタマー / 3'非翻訳配列 |
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| Outline of Final Research Achievements |
Anti-cytokine antibody therapy has proven the importance of cytokines as therapeutic targets. In this study, cytokines that regulate the metabolism of mRNA via AU rich element present in the 3 'untranslated region of mRNA were screened by using a reporter plasmid.
IL-1β enhanced the luciferase activity of the reporter having the 3 'UTR of CXCL2 mRNA. IL-1β leads to enhanced mRNA and protein expression of CXCL2 in synovial cells, which proved that CXCL2 mRNA is stabilized by IL-1β. Co-transfection of TTP with the reporter plasmid suppressed the luciferase activity with the 3′UTR of CXCL2 mRNA. It became clear that cytokine contributes to induction of an inflammatory gene by stabilizing the mRNA in addition to transcriptional up-regulation of the gene.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Upregulation of Thrombospondin 1 Expression in Synovial Tissues and Plasma of Rheumatoid Arthritis: Role of Transforming Growth Factor-beta1 toward Fibroblast-like Synovial Cells2015
Author(s)
Suzuki T, Iwamoto N, Yamasaki S, Nishino A, Nakashima Y, Horai Y, Kawashiri S, Y Ichinose K, Arima K, Tamai M, Nakamura H, Origuchi T, Miyamoto C, Osaki M, Ohyama K, Kuroda N, Kawakami A.
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Journal Title
Journal of Rheumatology
Volume: 42
Issue: 6
Pages: 943-947
DOI
Related Report
Peer Reviewed
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