The role of protein citrullination in antigen-induced airway inflammation

• Project/Area Number: 26461485
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Chiba University
• Principal Investigator: MAEZAWA Yuko 千葉大学, 医学部附属病院, 医員 (00724923)
• Co-Investigator(Kenkyū-buntansha): 中島 裕史 千葉大学, 大学院医学研究院, 教授 (00322024) ; 高取 宏昌 千葉大学, 医学部附属病院, 助教 (30568225)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アレルギー性気道炎症 / アレルギー性炎症 / シトルリン化

Outline of Final Research Achievements

Protein citrullination is one of post-translational protein modifications mediated by peptidylarginine deiminase （PAD). PAD over-expression and resulting abnormal protein citrullination have been reported to be involved in various autoimmune inflammatory diseases and non-autoimmune inflammatory diseases. However, the roles of PAD and protein citrullination in the pathogenesis of allergic airway inflammation has not been fully elucidated. We analyzed the effect of PAD inhibitor on antigen-induced murine airway inflammation and clarified its suppressive effect on the disease phenotypes via regulating T cell functions. PAD inhibitor also improved the phenotypes of obesity model mouse, suggesting the involvement of citrullinated proteins in various inflammation-mediated diseases.