A role of MafB transcription factor in the host defense against Mycobacteriuml tuberculosis infection
Project/Area Number |
26461498
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Infectious disease medicine
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Research Institution | Yamagata University |
Principal Investigator |
ABE Shuichi 山形大学, 医学部, 非常勤講師 (40400543)
|
Co-Investigator(Renkei-kenkyūsha) |
SHIBATA Yoko 山形大学, 医学部, 講師 (60333978)
KIMURA Tomomi 山形大学, 医学部, 医員 (50536935)
|
Research Collaborator |
NEMOTO Takako
SATO Masamichi
SATO Kento
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | MafB / マクロファージ / 貧食能 / Fcgr3 / 貪食 / 貪食能 / BCG |
Outline of Final Research Achievements |
Alveolar macrophage plays important roles in host defense against Mycobacterium tuberculosis (TB) infection in the lungs, including Fc receptor-mediated phagocytosis of TB. MafB is a transcription factor that regulates a variety of functions of macrophages. In order to assess the role of MafB in the mechanisms of phagocytosis in macrophages, we established the MafB gene-silenced macrophage cell line by gene transduction of MafB-shRNA to the macrophage cell line RAW264.7 (RAW264.7-MafB-shRNA). Fc receptor-mediated phagocytosis was suppressed by MafB gene silencing in RAW264.7 cells. Fcgr3 expression was significantly decreased in RAW264.7-MafB-shRNA compared to control cells. Forced expression of the Fcgr3 gene recovered Fc receptor-mediated phagocytosis in RAW264.7-MafB-shRNA. Our data suggested that MafB transcription factor should promote cell surface Fcgr3 expression and regulate Fc receptor-mediated phagocytosis in macrophages.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression.2017
Author(s)
Nemoto T, Shibata Y, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Sato M, Sato K, Nakano H, Abe S, Nishiwaki M, Kubota I.
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Journal Title
Biochem Biophys Res Commun.
Volume: 482
Issue: 2
Pages: 375-381
DOI
Related Report
Peer Reviewed
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[Journal Article] Pulmonary macrophage transplantation therapy2014
Author(s)
Suzuki T, Arumugam P, Sakagami T, Lachmann N, Chalk C, Sallese A, Abe S, Trapnell C, Carey B, Moritz T, Malik P, Lutzko C, Wood RE, Trapnell BC
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Journal Title
Nature
Volume: 514
Issue: 7523
Pages: 450-454
DOI
Related Report
Peer Reviewed
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