Tracking of microglial development and differentiation in the developing brain
| Project/Area Number |
26461556
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SAKUMA Hiroshi 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, プロジェクトリーダー (50425683)
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| Research Collaborator |
IGARASHI Ayuko
SAIKA Reiko
SUZUKI Tomonori
SHIMA Taiki
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ミクログリア / アストロサイト / インターロイキン34 / P2Y12受容体 / NLRP3 インフラマソーム / ADP / NF-κB / NLRP3インフラマソーム / 骨髄未分化細胞 / IL-34 / TGFβ / Time-lapse imaging / 造血幹細胞 / 前駆細胞 |
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| Outline of Final Research Achievements |
Development and differentiation of microglia in the developing brain were studied using in vitro co-culture model. After microglial depletion using CD11b-conjugated microbes, the number of microglia rapidly increased. When bone marrow lineage-negative cells were co-cultured with astrocytes, they differentiated into microglia-like cells with assists of IL-34 and TGFβ. Microglial P2Y12 purinergic receptor played pivotal role in the activation of NLRP inflammasome.
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Report
(5 results)
Research Products
(9 results)
