Clinical and bacteriological analysis of nontypeable Haemophilus influenzae isolated from invasive diseases
Project/Area Number |
26461564
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Chiba University |
Principal Investigator |
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Research Collaborator |
HOSHINO Tadashi
HISHIKI Haruka
NAITO Sachiko
TAKEUCHI Noriko
OHKUSU Misako
SEGAWA Shunsuke
TSUCHIDA Sachio
SATOH Mamoru
TAKAHASHI Hiroki
NISHI Junichiro
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | インフルエンザ菌 / Hibワクチン / 無莢膜株 / 薬剤耐性 / MLST解析 / 侵襲性感染症 / 非b型株 / 血清型解析 / 侵襲性感染症由来インフルエンザ菌 / インフルエンザ菌無莢膜株 / インフルエンザ菌e型 / バイオフィルム産生能 / インフルエンザ菌f型 ST124 / 侵襲性感染症由来インフルエンザ菌無莢膜株 / PFGE解析 / 血清殺菌能 / 細胞通過能 |
Outline of Final Research Achievements |
After the introduction of Haemophilus influenzae type b (Hib) vaccine, invasive H. influenzae infection is now mainly due to nontypeable H. influenzae (NTHi). We investigated the clinical characteristics of invasive NTHi disease in children by clarifying the pathogenicity of NTHi strains isolated from invasive diseases. Most of the strains were biotype II or III. Two β-lactamase positive ampicillin-clavulanate resistant strains were found. Other penicillin non-susceptible strains had multiple amino acid substitutions. The average of biofim formation of hia or pilli positive strains were higher than negative strains. IS1016 was positive in 10.7% of the tested strains. Invasive NTHi strains from our study showed a wide variety of characteristics. Continuous monitoring of the NTHi responsible for invasive diseases in children is important to detect the changes in the epidemiology of invasive H. influenzae infection in the post Hib vaccine era.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Analysis of Haemophilus influenzae serotype f isolated from three Japanese children with invasive H. influenzae infection.2015
Author(s)
Hoshino T, Hachisu Y, Kikuchi T, Tokutake S, Okui H, Kutsuna S, Fukasawa C, Murayama K, Oohara A, Shimizu H, Ito M, Takahashi Y, Ishiwada N.
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Journal Title
J Med Microbiol
Volume: E-pub ahead of print
Issue: 4
Pages: 355-358
DOI
Related Report
Peer Reviewed
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[Journal Article] Nationwide population-based surveillance of invasive pneumococcal disease in Japanese children: Effects of the seven-valent pneumococcal conjugate vaccine.2015
Author(s)
Suga S, Chang B, Asada K, Akeda H, Nishi J, Okada K, Wakiguchi H, Maeda A, Oda M, Ishiwada N, Saitoh A, Oishi T, Hosoya M, Togashi T, Oishi K, Ihara T.
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Journal Title
Vaccine
Volume: 33
Issue: 45
Pages: 6054-6060
DOI
Related Report
Peer Reviewed
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[Journal Article] The incidence of pediatric invasive Haemophilus influenzae and pneumococcal disease in Chiba prefecture, Japan before and after the introduction of conjugate vaccines.2014
Author(s)
Ishiwada N, Hishiki H, Nagasawa K, Naito S, Sato Y, Chang B, Sasaki Y, Kimura K, Ohnishi M, Shibayama K.
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Journal Title
Vaccine
Volume: 32
Issue: 42
Pages: 5425-5431
DOI
Related Report
Peer Reviewed
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