Classification of ependymoma of childhood and establishment of molecular diagnositic procedure for molecular target therapy of ependymoma.

• Project/Area Number: 26461573
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Matsumoto Dental University
• Principal Investigator: Shiohara Masaaki 松本歯科大学, 歯学部, 教授 (00293514)
• Co-Investigator(Kenkyū-buntansha): 重村 倫成 信州大学, 学術研究院医学系, 助教 (70623916)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 上衣腫 / SNA array / SNP array

Outline of Final Research Achievements

SNP array analysis using 13 samples of childhood ependymoma revealed that they have 1q gain (46%), 2q gain (31%), and 5q loss (15%), respectively. These copy number variations (CNV) are comfirmed by FISH analysis, except for 1 sample of 5q loss. The relationship between these CNV revealed by SNP array and clinical prognosis of ependymoma are not revealed. The relationship between the results of immunostaining of samples (nestin、tenasin-C、NELL2、LAMA2、and KIF27) and clinical prognosis of ependymoma are not revealed, either.