Study of recurrence mechanism in super high-risk neuroblastoma
| Project/Area Number |
26461591
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Tomoko Iehara 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (20285266)
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| Co-Investigator(Kenkyū-buntansha) |
細井 創 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20238744)
桑原 康通 京都府立医科大学, 医学(系)研究科(研究院), 講師 (30590327)
菊地 顕 京都府立医科大学, 医学(系)研究科(研究院), 特任助教 (40453104)
宮地 充 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40584983)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 神経芽腫 / スーパーハイリスク / ハイリスク / ｽｰﾊﾟｰﾊｲﾘｽｸ / 再発 |
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| Outline of Final Research Achievements |
Genome analysis was performed from the initial tumor and metastatic recurrent tumor tissue of the super high-risk group neuroblastoma. Deletion of CDKN2A, which does not exist at the onset, was observed in metastatic recurrent tissues. We analyzed CDKN2A and Rsf-1 in 19 neuroblastoma cell lines by real-time PCR method, there is no correlation was found with expression of CDKN2A and expression of Rsf-1. However, expression of Rsf-1 tended to be higher in the MYCN-amplified cell line. Next, We analyzed Segmental chromosome Aberration (SCA), when Rsf-1 knockdown was performed using the neuroblastoma cell line SH-SY5Y with high expression of Rsf-1. We cannot observed any changes in SCA (Segmental chromosome Aberration) in this cell line.
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Report
(4 results)
Research Products
(15 results)
