Analysis of Regenerated Bone Marrow by Adipose-derived stromal cells grown on a hydroxyapatite scaffold

• Project/Area Number: 26461595
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nippon Medical School
• Principal Investigator: UEDA TAKAHIRO 日本医科大学, 医学部, 准教授 (20322505)
• Co-Investigator(Kenkyū-buntansha): 右田 真 日本医科大学, 医学部, 助教授 (50256963)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 間葉系幹細胞 / 脂肪由来間葉系幹細胞 / 人工骨髄 / 造血幹細胞 / 人口骨髄 / 血管炎 / 幹細胞 / 再生医療 / MSC

Outline of Final Research Achievements

Regenerated bone marrow created using mouse adipose-derived mesenchymal cells (ADSC) was confirmed to be able to maintain long-term hematopoietic cells subcutaneously in NOD immunodeficient mice and was demonstrated to have the ability to provide bone marrow niches.　Moreover, the results of an investigation using a murine model of coronary vasculitis to confirm the anti-inflammatory effect of murine ADSC　revealed anti-inflammatory effects and　immune regulatory functions such as inhibition of inflammatory cytokines and reduction of the area of vasculitis; data useful for future elucidation of the mechanism of bone marrow reconstruction were obtained.

Research Products

• [Journal Article] 小球性貧血 (2017)
  • Author(s): 植田高弘
  • Journal Title: 小児科診療 Volume: ８０ Pages: 1163-1168
• [Journal Article] 小児白血病治療の現状と展望 (2015)
  • Author(s): 植田 高弘
  • Journal Title: 日本医科大学医学会雑誌 Volume: 11 Pages: 181-186
  • NAID: 130005111077
• [Journal Article] Adipose-derived stromal cells grown on a hydroxyapatite scaffold can support hematopoiesis in regenerated bone marrow in vivo. (2014)
  • Author(s): Ueda T, Fujita A, Ogawa R, Itoh Y, Fukunaga Y, Shimada T, MigitaM.
  • Journal Title: Cell Biologyl International Volume: ３８ Issue: 6 Pages: 790-798
  • DOI: 10.1002/cbin.10254
  • Peer Reviewed / Open Access