Morphological diagnosis of injured podocytes in various renal diseases with proteinuria by low vacuum scanning electron microscopy

• Project/Area Number: 26461610
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Tottori University
• Principal Investigator: okada shinichi 鳥取大学, 医学部, 准教授 (50343281)
• Co-Investigator(Renkei-kenkyūsha): INAGA Sumire 鳥取大学, 医学部, 助教 (60116358)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 蛋白尿 / 糸球体上皮細胞 / 低真空走査型電子顕微鏡 / 糸球体基底膜 / シクロスポリン / アルポート症候群 / 菲薄基底膜病

Outline of Final Research Achievements

We observed renal biopsy paraffin sections of various renal diseases patients by low vacuum scanning electron microscopy (LVSEM). The glomerular basement membranes showed characteristic coarse meshwork appearances in AS, and thin and sheet-like appearances in TBMN. Cyclosporin improved changes of foot processes of podocytes and alterations of podocytes cell bodies. Round-shaped podocytes cell bodies possibly represent injuries of podocytes in various renal diseases.

Research Products

• [Journal Article] Morphological diagnosis of Alport syndrome and thin basement membrane nephropathy by low vacuum scanning electron microscopy. (2014)
  • Author(s): Okada S, Inaga S, Kitamoto K, Kawaba Y, Nakane H, Naguro T, Kaidoh T, Kanzaki S
  • Journal Title: Biomed Res (Tokyo) Volume: 35 Pages: 345-350
  • NAID: 130004701685
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 低真空走査電子顕微鏡による各種腎疾患における糸球体上皮細胞形態変化の検討 (2016)
  • Author(s): 岡田晋一
  • Organizer: 第51回日本小児腎臓病学会
  • Place of Presentation: ウインクあいち（愛知県名古屋市）
  • Year and Date: 2016-07-07
• [Presentation] Alport症候群の腎障害進行における糸球体上皮細胞の意義 (2015)
  • Author(s): 岡田晋一，稲賀すみれ，横山浩己，山田祐子，北本晃一，河場康郎，中根裕信，海藤俊行，神﨑晋
  • Organizer: 第50回日本小児腎臓病学会
  • Place of Presentation: 神戸国際会議場（神戸市）
  • Year and Date: 2015-06-18
• [Presentation] Morphological alterations of podocytes in Alport syndrome investigated by low vacuum scanning electron microscopy (2015)
  • Author(s): Shinichi Okada, Sumire Inaga, Yuko Yamada, Koichi Kitamoto, Yasuo Kawaba, Hironobu Nakane, Toshiyuki Kaidoh, Susumu Kanzaki
  • Organizer: 52nd ERA-EDTA Congress 2015
  • Place of Presentation: London, UK
  • Year and Date: 2015-05-28
  • Int'l Joint Research
• [Presentation] 低真空走査型電子顕微鏡を用いたAlport症候群と菲薄基底膜病の鑑別 (2014)
  • Author(s): 岡田晋一、稲賀すみれ、北本晃一、河場康郎
  • Organizer: 第57回日本腎臓学会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-07-04 – 2014-07-06
• [Presentation] 低真空走査型電子顕微鏡を用いたAlport症候群の新規簡易診断法 (2014)
  • Author(s): 岡田晋一、稲賀すみれ、山田祐子、北本晃一 、河場 康郎、中根裕信、名黒知徳、海藤俊行 、神﨑 晋
  • Organizer: 第49回日本小児腎臓病学会
  • Place of Presentation: 秋田ビューホテル（秋田市）
  • Year and Date: 2014-06-05 – 2014-06-07