| Project/Area Number |
26461622
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSAKA Taiji 東海大学, 医学部, 教授 (50317749)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 先天性腎尿路異常 / 水腎症 / Keap1 / Nrf2 / 抗酸化防御機構 / Cre-loxシステム / コンディショナルターゲティング / 酸化ストレス |
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| Outline of Final Research Achievements |
In our previous observation, hydronephrosis was not infrequently seen in Keap1 knockdown mice in which Keap1 expression was partially suppressed. In this study, we expected that renal morphogenesis might be severely impaired in Keap1 knockout mice in which Keap1 is completely inactivated. However, the kidneys in Keap1 knockout mice at the age of day 10 did not show any morphological abnormality except for rather reduced size, which was compatible to the reduced body weight. As previously reported, Keap1 knockout mice could not survive beyond the weaning stage. So, we generated a conditionally targeted mouse in which Keap1 is inactivated only in the renal proximal straight tubule to enable observation in much later phase of the kidney morphology. At 32 weeks of age, the conditionally knockout mice did not show any renal morphological abnormalities including hydronephrosis and cortical/medullary cysts. In conclusion, Keap1 plays only a limited role in the process of renal morphogenesis.
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