Cell signaling pathway in pemphigus.

• Project/Area Number: 26461659
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kawasaki Medical School (2015-2016); Okayama University (2014)
• Principal Investigator: Aoyama Yumi 川崎医科大学, 医学部, 教授 (90291393)
• Research Collaborator: Kamiya Koji 自治医科大学, 皮膚科, 講師 ; Fujii Kazuyasu 鹿児島大学, 医学部・皮膚科学教室, 講師 (70452571) ; Kondo T. 国立がん研究センター研究所, 希少がん研究分野, 分野長 (30284061) ; Yamaguchi Mari 岡山大学, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 天疱瘡 / デスモグレイン３抗体 / 自己免疫 / デスモグレイン / プラキンファミリー / 病因性抗体 / 薬疹 / シグナル伝達 / プロテオミクス / 病因性エピトープ

Outline of Final Research Achievements

We have performed differential proteomic analysis of cultured keratinocyte stimulated with pathogenic/non-pathogenic anti-desmoglein3 antibody. Proteomic analysis and westernblotting analysis showed a significant enrichment of biological processes related to cell attachment, cytoskeleton organization, and ribosomal biogenesis.
We established novel method to detect pathogenic anti-Dsg3 antibody. The conformational Dsg3 ELISA index reflected the pathogenicity of anti-Dsg3 antibodies more accurately than the conventional Dsg3 ELISA index. Using both conventional and EDTA-treated ELISAs would be useful in monitoring the disease activity of PV.

Research Products

• [Journal Article] Epitope analysis of antidesmoglein 1 autoantibodies from patients with pemphigus foliaceus across different activity stages. (2016)
  • Author(s): Kamiya K, Aoyama Y, Yamasaki O, Kamata A, Yamagami J, Iwatsuki K, Tokura Y.
  • Journal Title: British Journal of Dermatology Volume: 174 Pages: 113-119
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Longitudinal analysis of antibody profiles against plakins in severe drug eruptions: emphasis on correlation with tissue damage in drug induced hypersensitivity syndrome and drug reaction with eosinophilia and systemic symptoms (2016)
  • Author(s): Takehara, A; Aoyama, Y; Kurosawa, M; Shirafuji, Y; Umemura, H; Kamiya, K; Ushigome, Y; Kano, Y; Shiohara, T; Iwatsuki, K;
  • Journal Title: British Journal of Dermatology Volume: 175 Pages: 944-952
  • Peer Reviewed
• [Journal Article] Atypical pemphigus with immunoglobulin G autoantibodies against desmoglein 3 and desmocollin 3. (2016)
  • Author(s): Kamiya K, Aoyama Y, Wakashima C, Kudo T, Nakajima K, Sano S, Ishii N, Teye K, Hashimoto T, Iwatsuki K, Tokura Y.
  • Journal Title: J Dermatol. Volume: 43 Issue: 8 Pages: 429-431
  • DOI: 10.1111/1346-8138.12906
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Epitope analysis of antidesmoglein 1 autoantibodies from patients with pemphigus foliaceus across different activity stages. (2015)
  • Author(s): Kamiya K, Aoyama Y, Yamasaki O, Kamata A, Yamagami J, Iwatsuki K, Tokura Y.
  • Journal Title: Br J Dermatol. Volume: 174 Issue: 1 Pages: 113-119
  • DOI: 10.1111/bjd.14098
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clues to digagnosis for unusual mucosal pemphigus demonstrating undetectable anti-desmoglein 3 serum antibodies by routine tests. (2015)
  • Author(s): Kamiya K, Aoyama Y, Yamaguchi M, Ukida A, Mizuno-Ikeda K, Fujii K, Hamada T, Tokura Y and Iwatsuki K.
  • Journal Title: Journal of Dermatology Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Epitope reduction of anti-desmoglein 1 autoantibodies in pemphigus foliaceus patients sera during clinical stages. (2015)
  • Author(s): Yumi Aoyama, Koji Kamiya, Osamu Yamasaki, Ken Ishii, Aki Kamata, Jun Yamagami, Yoshiki Tokura , Keiji Iwatsuki
  • Organizer: The 40th Annual Meeting of the Japanese Society for Investigative Dermatology.
  • Place of Presentation: Okayama, Japan
  • Year and Date: 2015-12-11
  • Int'l Joint Research
• [Presentation] Detection of antibodies against conformational epitopes of desmoglein in pemphigus and their pathogenicity (2015)
  • Author(s): Yumi Aoyama
  • Organizer: Pemphigus meeting 2015
  • Place of Presentation: Paris, France
  • Year and Date: 2015-09-07
  • Int'l Joint Research / Invited