| Project/Area Number |
26461659
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kawasaki Medical School (2015-2016)
Okayama University (2014) |
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Principal Investigator |
Aoyama Yumi 川崎医科大学, 医学部, 教授 (90291393)
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| Research Collaborator |
Kamiya Koji 自治医科大学, 皮膚科, 講師
Fujii Kazuyasu 鹿児島大学, 医学部・皮膚科学教室, 講師 (70452571)
Kondo T. 国立がん研究センター研究所, 希少がん研究分野, 分野長 (30284061)
Yamaguchi Mari 岡山大学, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 天疱瘡 / デスモグレイン３抗体 / 自己免疫 / デスモグレイン / プラキンファミリー / 病因性抗体 / 薬疹 / シグナル伝達 / プロテオミクス / 病因性エピトープ |
|---|
| Outline of Final Research Achievements |
We have performed differential proteomic analysis of cultured keratinocyte stimulated with pathogenic/non-pathogenic anti-desmoglein3 antibody. Proteomic analysis and westernblotting analysis showed a significant enrichment of biological processes related to cell attachment, cytoskeleton organization, and ribosomal biogenesis.
We established novel method to detect pathogenic anti-Dsg3 antibody. The conformational Dsg3 ELISA index reflected the pathogenicity of anti-Dsg3 antibodies more accurately than the conventional Dsg3 ELISA index. Using both conventional and EDTA-treated ELISAs would be useful in monitoring the disease activity of PV.
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