Development of the new cancer immunotherapy using iPS- derived CTLs for cancer stem cells
Project/Area Number |
26461923
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Wakayama Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
山上 裕機 和歌山県立医科大学, 医学部, 教授 (20191190)
中森 幹人 和歌山県立医科大学, 医学部, 准教授 (10322372)
尾島 敏康 和歌山県立医科大学, 医学部, 講師 (60448785)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | iPS細胞 / 樹状細胞 / 癌免疫療法 / adenovirus vector / 細胞傷害性T細胞 / 癌幹細胞 / 細胞障害性T細胞 |
Outline of Final Research Achievements |
We have reported an effective antitumor immune response of cytotoxic T lymphocytes (CTLs) by vaccine therapy using dendritic cells (DCs). Moreover, we have used the induced pluripotent stem (iPS) cell-derived DCs (iPSDCs). In the present study, we used carcinoembryonic antigen (CEA) of gastrointestinal cancers, and examined an actual antitumor effect using a CEA transgenic mouse model. We adenovirally transduced the CEA gene into mouse iPSDCs (miPSDCs) and immunized mice once with the genetically modified DCs. The cytotoxic activity of CTLs and the therapeutic efficacy of this vaccination were assayed. Our results showed significantly higher cytotoxicity against MC38-CEA and significantly higher therapeutic efficacy in mice administered with miPSDCs-CEA than in mice immunized with PBS and miPSDCs-LacZ. Therefor, this vaccination strategy using genetically modified iPSDCs expressing CEA may be useful for future clinical application against patients with a gastrointestinal cancer.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Circular stapling versus triangulating stapling for the cervical esophagogastric anastomosis after esophagectomy in patients with thoracic esophageal cancer: A prospective, randomized, controlled trial.2017
Author(s)
Hayata K, Nakamori M, Nakamura M, Ojima T, Iwahashi M, Katsuda M, Tsuji T, Kato T, Kitadani J, Takeuchi A, Tabata H, Yamaue H.
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Journal Title
Surgery.
Volume: in press
Issue: 1
Pages: 131-138
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Expression of BRCA1, a factor closely associated with relapse-free survival, in patients who underwent neoadjuvant chemotherapy with docetaxel, cisplatin, and fluorouracil for squamous cell carcinoma of the esophagus.2017
Author(s)
Ojima T, Nakamori M, Nakamura M, Katsuda M, Hayata K, Nakamura Y, Yamaue H.
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Journal Title
Surg Today
Volume: 47(1)
Pages: 65-73
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Cancer Vaccine Therapy using iPS-derived Dendritic Cells2016
Author(s)
Ojima T, Iwamoto H, Kitadani J, Tabata H, Nakamori M, Nakamura M, Katsuda M, Hayata K, Kato T, Takeuchi A, Yamaue H.
Organizer
第54回日本癌治療学会学術集会
Place of Presentation
横浜
Year and Date
2016-10-20
Related Report
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[Presentation] Cancer Vaccine Therapy using iPS-derived Dendritic Cells2016
Author(s)
Ojima T, Iwamoto H, Kitadani J, Tabata H, Nakamori M, Nakamura M, Katsuda M, Hayata K, Kato T, Takeuchi A, Yamaue H.
Organizer
第25回日本癌病態治療研究会
Place of Presentation
千葉
Year and Date
2016-06-08
Related Report
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