Clinical study on the protective effect of ischemic preconditioning against spinal cord injury

• Project/Area Number: 26462110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Yamaguchi University
• Principal Investigator: TAKAHASHI Masaya 山口大学, 医学部附属病院, 助教 (60634730)
• Co-Investigator(Kenkyū-buntansha): 細山 徹 山口大学, 医学(系)研究科(研究院), 助教 (20638803) ; 李 桃生 長崎大学, 原爆後障害医療研究所, 教授 (50379997)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 虚血プレコンディショニング / 脊髄虚血保護 / 虚血プレコンディショニング法 / CD34陽性骨髄細胞 / 虚血耐性

Outline of Final Research Achievements

GDF15 is known as one of ischemia-reperfusion protective factors. The GDF15 expression level in CD34-positive bone marrow cells was higher compared to CD34-negative bone marrow cells and PBMNC in C57BL/6 mice. Ischemic preconditioning was performed in abdominal aorta of mice. MicroRNAs was extracted from plasma exosomes at 24 hour after IPC. MicroRNA microarray analyses were performed using miRNA Oligo chip. IPC was performed for patients undergoing thoracic stent graft. MicroRNAs was extracted from serum exosomes before surgery and at 24 hour after IPC. MicroRNA microarray analyses were performed using miRNA Oligo chip.　Expression level of one microRNA targeting ANGPT1 was downregulated in IPC treated patients undergoing thoracic stent graft.

Research Products

• [Journal Article] Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts (2016)
  • Author(s): Ueno K, Takeuchi Y, Samura M, Tanaka Y, Nakamura T, Nishimoto A, Murata T, Hosoyama T, Hamano K
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 28538-28538
  • DOI: 10.1038/srep36758
  • Peer Reviewed / Open Access
• [Presentation] 虚血プレコンディショニングによる脊髄障害予防の機序解析 (2017)
  • Author(s): 溝口 高弘、上野 耕司, 竹内 由利子，佐村 誠, 細山 徹, 森景 則保, 美甘 章仁, 濱野 公一
  • Organizer: 第47回日本心臓血管外科学会
  • Place of Presentation: 東京 グランドニッコー東京 台場
  • Year and Date: 2017-02-27