INTRAVENOUS INFUSION OF MESENCHYMAL STEM CELLS PROMOTES FUNCTIONAL RECOVERY IN A MODEL OF CHRONIC SPINAL CORD INJURY

• Project/Area Number: 26462213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Sapporo Medical University
• Principal Investigator: Morita Tomonori 札幌医科大学, 医学部, 研究員 (60723343)
• Co-Investigator(Kenkyū-buntansha): 佐々木 祐典 札幌医科大学, 医学部, 講師 (20538136) ; 中崎 公仁 札幌医科大学, 医学部, 研究員 (70722461) ; 竹林 庸雄 札幌医科大学, 医学部, 准教授 (30347166) ; 鰐渕 昌彦 札幌医科大学, 医学部, 准教授 (30343388) ; 山下 敏彦 札幌医科大学, 医学部, 教授 (70244366) ; 押切 勉 札幌医科大学, 医学部, 研究員 (70754612)
• Co-Investigator(Renkei-kenkyūsha): HONMOU Osamu 札幌医科大学, 医学部, 教授 (90285007)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: mesenchymal stem cell / spinal cord injury / transplantation / 脊髄損傷 / 機能回復 / 骨髄幹細胞 / 慢性期

Outline of Final Research Achievements

Intravenous infusion of mesenchymal stem cells (MSCs) derived from bone marrow improves behavioral function in rat models of acute spinal cord injury (SCI). In this study, MSCs were infused 10 weeks after the induction of a severe contusive SCI. Locomotor function was assessed until 20 weeks post-SCI. Motor recovery was greater in the MSC group with rapid improvement beginning in earlier post-infusion times than in the vehicle group. Blood spinal cord barrier (BSCB) integrity was assessed by the intravenous infusion of Evans Blue with quantitation of its leakage, and that was reduced in the MSC group. There was extensive remyelination around the lesion center and increased neovascularization/sprouting of the nerve fibers. These results indicate that the MSC therapy results in functional improvements that is associated with structural changes in the chronically injured spinal cord including stabilization of the BSCB, axonal sprouting/regeneration and remyelination.

Research Products

• [Journal Article] INTRAVENOUS INFUSION OF MESENCHYMAL STEM CELLS PROMOTES FUNCTIONAL RECOVERY IN A MODEL OF CHRONIC SPINAL CORD INJURY (2016)
  • Author(s): Tomonori Morita, Masanori Sasaki, Yukon Kataoka-Sasaki, Masahito Nakazaki, Hiroshi Nagahama, Shinichi Oka, Tsutomu Oshigiri, Tsuneo Takebayashi, Toshihiko Yamashita, Jeffery D. Kocsis, Osamu Honmou
  • Journal Title: Neuroscience Volume: 335 Pages: 221-231
  • DOI: 10.1016/j.neuroscience.2016.08.037
  • NAID: 40021725590
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] INTRAVENOUS INFUSION OF MESENCHYMAL STEM CELLS PROMOTES FUNCTIONAL RECOVERY IN A MODEL OF CHRONIC SPINAL CORD INJURY (2017)
  • Author(s): Tomonori Morita, Masanori Sasaki, Yukon Kataoka-Sasaki, Masahito Nakazaki, Hiroshi Nagahama, Shinichi Oka, Tsutomu Oshigiri, Tsuneo Takebayashi, Toshihiko Yamashita, Jeffery D. Kocsis, Osamu Honmou
  • Organizer: Orthopaedic Research Society 2017 Annual Meeting
  • Place of Presentation: San Diego
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] ラット慢性期脊髄損傷モデルに対する骨髄間葉系幹細胞の経静脈的投与による機能回復の検討 (2016)
  • Author(s): 森田智慶, 佐々木祐典, 佐々木優子, 中崎公仁, 長濱宏史, 岡真一, 押切勉, 竹林庸雄, 山下敏彦, 本望修
  • Organizer: 第131回北海道整形災害外科学会
  • Place of Presentation: 函館
  • Year and Date: 2016-06-04
• [Presentation] ラット慢性期脊髄損傷モデルに対する骨髄間葉系幹細胞の経静脈的投与による機能回復の検討 (2016)
  • Author(s): 森田智慶
  • Organizer: 第１３１回北海道整形災害外科学会
  • Place of Presentation: 函館アリーナ（北海道函館市）
  • Year and Date: 2016-06-04