Development of a new treatment method of intractable fractures using carbon dioxide

• Project/Area Number: 26462266
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: NIIKURA Takahiro 神戸大学, 医学部附属病院, 特命講師 (40448171)
• Co-Investigator(Kenkyū-buntansha): 李 相亮 神戸大学, 医学部附属病院, 助教 (40533732) ; 岩倉 崇 神戸大学, 医学研究科, 医学研究員 (60437473)
• Research Collaborator: KUROIWA Yu ; ARAKURA Michio ; TAKAHARA Syunsuke
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 骨折治癒促進 / 難治性骨折 / 炭酸ガス / 骨再生 / 骨欠損

Outline of Final Research Achievements

We conducted an animal study as a pre-clinical trial to develop a new treatment method of an intractable fractures. Bone defect was created in the rat femoral shaft and stabilized with external fixator. After the surgery to create bone defect model, the healing process was compared between a group with transcutaneous carbon dioxide absorption and a control group without it. Bone healing was assessed radiographically and histologically. The bone mineral density of the newly formed bone was measured. The mechanical properties of the excised femurs were assessed. We found that bone healing was accelerated through an acceleration of endochondral ossification by the transcutaneous carbon dioxide absorption. Our results suggested that the transcutaneous carbon dioxide absorption could be a beneficial treatment option of intractable fractures.

Research Products

• [Presentation] Transcutaneous CO2 Application Accelerates Bone Regeneration in a Rat Femoral Defect Model (2017)
  • Author(s): Yu Kuroiwa, Takahiro Niikura, Shunsuke Takahara, Sang Yang Lee, Takashi Iwakura, Michio Arakura, Yohei Kumabe, Yoshitada Sakai, Ryosuke Kuroda
  • Organizer: The 63rd Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] 炭酸ガス経皮吸収の 継続期間がラット骨折治癒に与える影響の検討 (2016)
  • Author(s): 岩倉崇、新倉隆宏、李相亮、高原俊介、新倉路生、黒田良祐、黒坂昌弘
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2016-10-13
• [Presentation] 炭酸ガスを利用した新しい骨折治癒促進法の臨床試験 中間報告 (2015)
  • Author(s): 新倉 隆宏, 李 相亮, 岩倉 崇, 黒坂 昌弘
  • Organizer: 第41回日本骨折治療学会
  • Place of Presentation: 奈良市
  • Year and Date: 2015-06-26
• [Presentation] 炭酸ガスを利用した新しい偽関節治療 (2015)
  • Author(s): 新倉 隆宏, 李 相亮, 岩倉 崇, 黒坂 昌弘
  • Organizer: 第124回中部日本整形外科災害外科学会
  • Place of Presentation: 金沢市
  • Year and Date: 2015-04-10
• [Presentation] The Effects of the Duration of Transcutaneous CO2 Application on the Facilitatory Effect in Rat Fracture Repair (2015)
  • Author(s): Takashi Iwakura, Takahiro Niikura, Sang Yang Lee, Etsuko Okumachi, Takahiro Waki, Syunsuke Takahara, Michio Arakura, Yoshitada Sakai, Ryosuke Kuroda, Masahiro Kurosaka
  • Organizer: The 61th Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] 炭酸ガス経皮吸収の継続期間が骨折治癒に与える影響 (2015)
  • Author(s): 岩倉崇、新倉隆宏、李相亮、黒田良祐、黒坂昌弘
  • Organizer: 第28回日本創外固定・骨延長学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-20 – 2015-03-21
• [Presentation] 炭酸ガス経皮吸収の期間が骨折治癒促進作用に与える影響の検討 (2014)
  • Author(s): 岩倉崇、新倉隆宏、李相亮、堂垣佳宏、奥町悦子、脇貴洋、高原俊介、黒坂昌弘
  • Organizer: 第40回日本骨折治療学会
  • Place of Presentation: 熊本市
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] 骨折治療への新たなアプローチ 炭酸ガス経皮吸収による骨折治癒促進 (2014)
  • Author(s): 新倉隆宏、 李相亮、 岩倉崇、 黒坂昌弘
  • Organizer: 第87回日本整形外科学会学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2014-05-22 – 2014-05-25