| Project/Area Number |
26462267
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
KUBO Tadahiko 広島大学, 医歯薬保健学研究院(医), 准教授 (70397959)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
越智 光夫 広島大学, その他部局等, 学長 (70177244)
下瀬 省二 独立行政法人国立病院機構(呉医療センター臨床研究部), その他部局等, 医師 (30304439)
藤森 淳 広島大学, 医歯薬保健学研究院(医), 寄附講座助教 (70632256)
坂口 剛正 広島大学, 医歯薬保健学研究院(医), 教授 (70196070)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | 骨肉腫 / 蛍光イメージング / 腫瘍溶解性ウイルス / 近赤外線蛍光蛋白 |
|---|
| Outline of Final Research Achievements |
Recombinant VSV expressing katushka was generated using the established method of reverse genetics. Five to six weeks old C3H mice were implanted subcutaneously with 1x107 osteosarcoma cells LM8 on the right hind leg. We observed tumor-specific fluorescence in vitro using fluorescence microscopy（BZ-9000、keyence）and in osteosarcoma-bearing mice using Bio-fluorescence imaging system （WinLight32, Berthold）. Using this imaging system, tumor wide resection was performed safely and precisely and circulating tumor cells were eliminated securely. In conclusion, this study proved the possibility of the new fluorescence imaging system to apply to the advanced therapy of primary lesion and circulating tumor cells.
|
