Establishment of novel osteochondral allografting combined with growth factor- collagen-binding domain fusion technology
| Project/Area Number |
26462277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
Onuma Kenji 北里大学, 医学部, 講師 (80348557)
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| Co-Investigator(Kenkyū-buntansha) |
内田 健太郎 北里大学, 医学部, 講師 (50547578)
齋藤 亘 北里大学, 医学部, 助教 (60439099)
井上 玄 北里大学, 医学部, 准教授 (80594209)
占部 憲 北里大学, 北里大学メディカルセンター, 教授 (90284489)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSHITA Osamu 岡山大学, 医歯(薬)学総合研究科, 教授 (00209537)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 同種骨軟骨移植 / 成長因子固相化技術 / 成長因子固相化 / 組織保存 / 同種移植 / 骨軟骨移植 / 同種骨軟骨組織移植 / コラーゲン結合蛋白 / 成長因子 |
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| Outline of Final Research Achievements |
We investigated study for improved osteochondral allografting combined with basic fibroblast growth factor (bFGF)- collagen-binding domain (CBD) fusion protein. In the preliminary study, optimal reaction time for binding bFGF-CBD to rat osteochondral tissue (OCT) samples was 15 minutes. Cylindrical OCTs were harvested from rabbit femoral condyles and then preserved for 1 week in the preservation solutions at 4 ℃. Then OCTs were reacted to bind bFGF-CBD for 15 minutes in the solution containing bFGF-CBD and then implanted to rabbit osteochondral defect models. While in the control samples, OCTs were not bound with bFGF-CBD. At 4 and 8 weeks after implantation, condyles were harvested and then samples were accessed using micro-CT and histological samples stained with HE and safranin-o. We could not observed significant difference between OCT samples with bFGF-CBD and control samples. These results concluded that bFGF-CBD might not improve osteochondral allografting of rabbit model.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Activation of calcitonin gene-related peptide signaling through the prostaglandin E2-EP1/EP2/EP4 receptor pathway in synovium of knee osteoarthritis patients.2016
Author(s)
Atsushi Minatani, Kentaro Uchida, Gen Inoue, Shotaro Takano, Jun Aikawa, Masayuki Miyagi, Hisako Fujimaki, Dai Iwase, Kenji Onuma, Toshihide Matsumoto, Masashi Takaso
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Journal Title
Journal of orthopaedic surgery and reserch
Volume: -
Issue: 1
Pages: 117-117
DOI
Related Report
Peer Reviewed / Open Access
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