| Project/Area Number |
26462277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
Onuma Kenji 北里大学, 医学部, 講師 (80348557)
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| Co-Investigator(Kenkyū-buntansha) |
内田 健太郎 北里大学, 医学部, 講師 (50547578)
齋藤 亘 北里大学, 医学部, 助教 (60439099)
井上 玄 北里大学, 医学部, 准教授 (80594209)
占部 憲 北里大学, 北里大学メディカルセンター, 教授 (90284489)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSHITA Osamu 岡山大学, 医歯(薬)学総合研究科, 教授 (00209537)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 同種骨軟骨移植 / 成長因子固相化技術 / 成長因子固相化 / 組織保存 / 同種移植 / 骨軟骨移植 / 同種骨軟骨組織移植 / コラーゲン結合蛋白 / 成長因子 |
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| Outline of Final Research Achievements |
We investigated study for improved osteochondral allografting combined with basic fibroblast growth factor (bFGF)- collagen-binding domain (CBD) fusion protein. In the preliminary study, optimal reaction time for binding bFGF-CBD to rat osteochondral tissue (OCT) samples was 15 minutes. Cylindrical OCTs were harvested from rabbit femoral condyles and then preserved for 1 week in the preservation solutions at 4 ℃. Then OCTs were reacted to bind bFGF-CBD for 15 minutes in the solution containing bFGF-CBD and then implanted to rabbit osteochondral defect models. While in the control samples, OCTs were not bound with bFGF-CBD. At 4 and 8 weeks after implantation, condyles were harvested and then samples were accessed using micro-CT and histological samples stained with HE and safranin-o. We could not observed significant difference between OCT samples with bFGF-CBD and control samples. These results concluded that bFGF-CBD might not improve osteochondral allografting of rabbit model.
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