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Development of a novel method to analyze cancer-derived circulating exosomes

Research Project

Project/Area Number 26462411
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionOsaka University

Principal Investigator

Uemura Motohide  大阪大学, 医学系研究科, 寄附講座准教授 (40631015)

Co-Investigator(Kenkyū-buntansha) 藤田 和利  大阪大学, 医学系研究科, 講師 (50636181)
Co-Investigator(Renkei-kenkyūsha) JINGUSHI Kentaro  大阪大学, 大学院薬学研究科・分子細胞生理学, 招へい教員 (80707571)
TSUJIKAWA Kazutake  大阪大学, 大学院薬学研究科・分子細胞生理学, 教授 (10207376)
NONOMURA Norio  大阪大学, 大学院医学系研究科・器官制御外科学講座(泌尿器科学), 教授 (30263263)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsエクソソーム / miRNA / 腎細胞癌 / マイクロRNA / マイクロアレイ解析 / 再発予測因子
Outline of Final Research Achievements

We aimed to develop novel methods for detecting cancer-specific exosomes. We cultured paired renal cancer tissues and matched adjacent non-cancerous kidney tissues using our novel methods and obtained extracellular vesicles (EVs) derived from the matched tissues using an ultracentrifuge method. We confirmed that the EVs collected by our methods were genuine exosomes in terms of their size and morphologic features. Then, comprehensive proteomics analysis was performed by Liquid Chromatography-tandem Mass Spectrometry (LC/MS/MS) for exosomes obtained from cultured medium and matched patient serum. Protein identification was performed by Sequest and Mascot search. We identified several exosomal proteins in patient serum that could serve as biomarkers for renal cancer. We have also issued a patent application (2016-211239) so that our methods can be applied for international use.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015

All Presentation (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Presentation] 腎癌特異的細胞外小胞(エクソソーム)の新規解析手法の開発と血液バイオマーカー探 索および治療への臨床応用2016

    • Author(s)
      植村元秀
    • Organizer
      第104回日本泌尿器科学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2016-04-23
    • Related Report
      2015 Research-status Report
  • [Presentation] microRNA-27a-3pは淡明細胞型腎細胞癌の予後規定因子である2015

    • Author(s)
      中田 渡
    • Organizer
      日本泌尿器科学会
    • Place of Presentation
      金沢市
    • Year and Date
      2015-04-18
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] バイオマーカーの探索方法、及び腎がんマーカー2016

    • Inventor(s)
      植田幸嗣、植村元秀、藤田和利ほか
    • Industrial Property Rights Holder
      植田幸嗣、植村元秀、藤田和利ほか
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-10-28
    • Related Report
      2016 Annual Research Report
    • Overseas

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Published: 2014-04-04   Modified: 2018-03-22  

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