| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Aberrant expression of miRNAs has been implicated in the progression and metastasis of cancer. The present study aimed to investigate whether miR-331-3p positively affects the epithelial-to-mesenchymal transition (EMT). Overexpression of miR-331-3p promoted cell migration and up-regulated mesenchymal markers such as vimentin, N-cadherin, and snail and down-regulated epithelial markers such as E-cadherin and desmoplakin in the prostate cancer cell line PC3. We identified NEP2 and NACC1 as putative target molecules in silico, as they were closely associated with the expression of miR-331-3p and TGF-β/Smad 4 signals. MiR-331-3p-mediated miRNA maturation and enhanced EMT via effects on TGF-β/Smad 4 are essential for the development of prostate cancer.
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