Tissue engineering for lower urinary tract using autologous bone marrow or fatty tissue derived stem cells.

• Project/Area Number: 26462441
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Shinshu University
• Principal Investigator: ISHIZUKA Osamu 信州大学, 学術研究院医学系, 教授 (20184541)
• Co-Investigator(Kenkyū-buntansha): 今村 哲也 信州大学, 医学部, 特任講師 (00467143)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 再生医療 / 膀胱 / 尿道括約筋 / 骨髄幹細胞 / 脂肪幹細胞 / 移植・再生医療 / 再生医学 / 細胞・組織 / 生体機能利用 / 脳神経疾患

Outline of Final Research Achievements

We could culture and proliferate the bone-marrow-derived mesenchymal stem cells derived from the femur of rabbit, or fatty-tissue derived stem cells to stem cell sheets. The cultured stem cell sheet were implanted into the radiation injured bladder or cryo-injured urethral sphincter, and we could found the reconstruction of the bladder and urethral sphincter by immuno-histological methods and functional study. This fact means stem cells sheet is more effetive for tissue engineering of lower urinary tract than simple stem cell injection methods.

Research Products

• [Journal Article] Implantation of autologous bone marrow-derived cells improves erectile dysfunction in spontaneously hypertensive rats (2017)
  • Author(s): Ishikawa, M., Imamura, T., Nishizawa, O., Ishizuka,O.
  • Journal Title: Shinshu Medical Journal Volume: 65 Pages: 37-44
  • NAID: 130005397650
  • Peer Reviewed
• [Journal Article] Engineered bone marrow-derived cell sheets restore structure and function of radiation-injured rat urinary bladder (2015)
  • Author(s): Imamura T, Ogawa T, Minagawa T, Yokoyama H, Nakazawa M, Nishizawa O, Ishizuka O
  • Journal Title: Tissue Engineering Part A Volume: 21 Issue: 9-10 Pages: 1600-1610
  • DOI: 10.1089/ten.tea.2014.0592
  • NAID: 120007100146
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Implantation of autologous adipose-derived cells reconstructs functional urethral sphincters in rabbit cryo-injured urethra. (2014)
  • Author(s): Sudha, G.S., Imamura, T., Ishizuka, O., Zhang, L., Yamagishi, T., Yokoyama, H., Minagawa, T., Ogawa, T., Kurizaki, Y., Kato, H., Nishizawa, O.
  • Journal Title: Tissue Eng. Part A. Volume: 20 Pages: 1971-9
  • NAID: 120007100147
  • Peer Reviewed / Open Access
• [Presentation] バイオ３Ｄプリンターで作製した骨髄由来細胞構造体による放射線障害膀胱の機能的な膀胱再生への試み (2017)
  • Author(s): 今村哲也、島村 満、畠山奈々美、ゴータム スダ、永井 崇、小川輝之、皆川倫範、齊藤徹一、鈴木都史郎、中沢昌樹、石塚 修
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2017-03-07
• [Presentation] 下部尿路再生をめざした新規細胞凝集体（スフェロイド）シート作製の試み (2015)
  • Author(s): 今村哲也、小川輝之、皆川倫範、永井 崇、横山 仁、石川雅邦、平形志朗、道面尚
  • Organizer: 第１０４回日本泌尿器科学会総会
  • Place of Presentation: ホテル日航金沢（石川県金沢市）
  • Year and Date: 2015-04-18
• [Presentation] 下部尿路再生をめざした新規細胞凝集体（スフェロイド）シート作製の試み (2015)
  • Author(s): 今村哲也、小川輝之、今村哲也、小川輝之、皆川倫範、永井 崇、横山 仁、石川雅邦、平形志朗、道面尚久、中沢昌樹、石塚 修
  • Organizer: 第１０４回日本泌尿器科学会総会
  • Place of Presentation: ホテル金沢
  • Year and Date: 2015-04-18
• [Presentation] Transplantation of cell sheets produced from bone marrow-derived cells reconstructs functional urinary bladders in radiation injury rat models. (2014)
  • Author(s): Imamura, T., Ishizuka, O., Ogawa, T., Yokoyama, H., Minagawa, M., Nakazawa, M., Nishizawa, O.
  • Organizer: The 44th Annual Meeting of the International Continence Society.
  • Place of Presentation: Rio de Janeiro, Brazil
  • Year and Date: 2014-10-20 – 2014-10-24
• [Presentation] Cell sheet produced by bone marrow-derived cells regenerate functional urinary bladders in rat radiation-injured bladder model. (2014)
  • Author(s): Imamura, T., Ishizuka, O., Ogawa, T., Minagawa, M., Yokoyama, H., Nakazawa, M., Kato,H., Nishizawa, O.
  • Organizer: The 9th Pan-Pacific Continece Society Meeting.
  • Place of Presentation: Taichung, Taiwan
  • Year and Date: 2014-09-12 – 2014-09-13