| Project/Area Number |
26462567
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
OKADA Hiroko 順天堂大学, 医学部, 助教 (20433774)
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| Co-Investigator(Kenkyū-buntansha) |
神谷 和作 順天堂大学, 医学部, 准教授 (10374159)
飯塚 崇 順天堂大学, 医学部, 非常勤助教 (40372932)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝性難聴 / GJB2 / コネキシン26 / 前庭 / トランスジェニックマウス / 遺伝子治療 / アデノ随伴ウィルス / 前庭機能 / Gjb2 |
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| Outline of Final Research Achievements |
We analyzed the morphological and functional development of the vestibular organ in Cx26 transgenic mice between 0 days after birth (P0) and P140, which was compared with that of littermate control mice (non-Tg). The gross structure of the inner ear in non-transgenic and transgenic mice revealed no hydrops, no defects, no degeneration in either the cochlea or the vestibule throughout the postnatal period. Light microscopic observations in the sensory epithelium revealed normally developed and matured utricle macula, saccular macula, and ampulla in the transgenic mice, which were very similar to those of the nontransgenic mouse. The present study clearly demonstrated that postnatal development and maturation in the vestibular organ were morphologically and functionally completed in Cx26 transgenic mice. Furthermore, we examined the gene transfer with adeno associated virus (AAV) via semicircular canal and successfully delivered AAV into the vestibular cells.
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