Identification of serum and salivary markers of head and neck cancers by mass spectrometer

• Project/Area Number: 26462595
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: Ogawa Takenori 東北大学, 大学病院, 講師 (50375060)
• Co-Investigator(Kenkyū-buntansha): 堀井 明 東北大学, 医学系研究科, 教授 (40249983) ; 齋木 由利子 東北大学, 医学系研究科, 助教 (80311223)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 頭頸部癌 / 血清マーカー / マイクロＲＮＡ / 質量分析計 / 癌 / トランスレーショナルリサーチ / マイクロアレイ

Outline of Final Research Achievements

Currently, no reliable serum biomarkers for head and neck squamous cell carcinoma exists as they do for serum SCC using Cyfra levels. The aim of this study is identification of new biomarkers using microRNA microarray and mass spectrometry. We compared normal mucosa to head and neck squamous cell carcinoma samples using MicroRNA analysis which allowed us to identify 8 microRNA candidates including those in the miR21 and miR200 families. These microRNA candidates were then validated using real time PCR. Two of the eight microRNA were identified in serum circulating microRNA. Further analysis of the microRNA candidates using mass spectrometry identified a new Galectin-3 binding protein serum marker. These new biomarkers may be useful in head and neck cancer diagnosis and treatment.

Research Products

• [Remarks] 東北大学病院 耳鼻咽喉・頭頸部外科学教室ホームページ
  • URL: http://www.orl.med.tohoku.ac.jp/