Epigenetic regulation of large intergenic non-coding RNA in Head and Neck Cancer

• Project/Area Number: 26462599
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Misawa Kiyoshi 浜松医科大学, 医学部附属病院, 講師 (90334979)
• Co-Investigator(Kenkyū-buntansha): 遠藤 志織 浜松医科大学, 医学部附属病院, 医員 (10625205) ; 峯田 周幸 浜松医科大学, 医学部, 教授 (40190714)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 頭頸部癌 / DNAメチル化 / SALL4遺伝子 / non-coding RNA / エピジェネティクス / エピジェネティックス

Outline of Final Research Achievements

This study examined non-coding RNA profiles of head and neck cancer (HNSCC) cell lines by Sal-like protein (SALL)4 siRNA transfection evaluated their significance and value as a biomarker.SALL4 siRNA transfection was associated with transcriptional inhibition of 3.8% lincRNA and was correlated with transcriptionalincrement of 3.6% lincRNA by next-generation sequencing analysis.

Research Products

• [Journal Article] "Site-specific methylation patterns of the GAL and GALR1/2 genes in head and neck cancer: potential utility as biomarkers for prognosis (2017)
  • Author(s): Misawa K, Mochizuki D, Endo S, Mima M, Misawa Y, Imai A, Shinmura K, Kanazawa T, Carey TE, Mineta H
  • Journal Title: Mol carcinogenesis Volume: 56(3) Issue: 3 Pages: 1107-1116
  • DOI: 10.1002/mc.22577
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Indwelling Voice Prosthesis insertion after Total Pharyngolaryngectomy with Free Jejunal Reconstruction (2017)
  • Author(s): Fukushima Hirofumi, Kanazawa Takeharu, Kawabata Kazuyoshi, Mitani Hiroki, Yonekawa Hiroyuki, Sasaki Toru, Shimbashi Wataru, Seto Akira, Kamiyama Ryousuke, Misawa Kiyoshi, Asakage Takahiro
  • Journal Title: Laryngoscope Investigative Otolaryngology Volume: 2(1) Issue: 1 Pages: 30-35
  • DOI: 10.1002/lio2.63
  • Peer Reviewed / Open Access
• [Journal Article] Human papillomavirus-associated small cell carcinoma/neuroendocrine carcinoma of the oropharynx: A report of two cases (2016)
  • Author(s): Misawa K, Kawasaki H, Matsuo R, Sugiyama K, Mochizuki D, Endo S, Imai A, Misawa Y, Yamatodani T, Mizuta K, Mineta H
  • Journal Title: SpringerPlus Volume: 5(1) Issue: 1 Pages: 1847-1847
  • DOI: 10.1186/s40064-016-3501-x
  • Peer Reviewed / Open Access
• [Journal Article] Late recurrence of breast carcinoma metastasis to the hypopharynx: a case report (2016)
  • Author(s): Izumi C, Misawa K, Endo S, Sugiyama K, Mochizuki D, Imai A, Mima M, Yamatodani T, Mineta H
  • Journal Title: SpringerPlus Volume: 5 Issue: 1 Pages: 599-599
  • DOI: 10.1186/s40064-016-2226-1
  • Peer Reviewed / Open Access
• [Journal Article] Abnormal Expressions of DNA Glycosylase Genes NEIL1, NEIL2, and NEIL3 Are Associated with Somatic Mutation Loads in Human Cancer. (2016)
  • Author(s): Shinmura K, Kato H, Kawanishi Y, Igarashi H, Goto M, Tao H, Inoue Y, Nakamura S, Misawa K, Mineta H, Sugimura H.
  • Journal Title: Oxidative Medicine and Cellular Longevity Volume: 2016 Issue: 1 Pages: 1546392-1546392
  • DOI: 10.1155/2016/1546392
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Aberrant methylation inactivates somatostatin and somatostatin receptor type 1 in head and neck squamous cell carcinoma. (2015)
  • Author(s): Misawa K, Misawa Y, Kondo H, Mochizuki D, Imai A, Fukushima H, Uehara T, Kanazawa T, Mineta H
  • Journal Title: PLoS ONE Volume: 3 Issue: 3 Pages: e0118588-e0118588
  • DOI: 10.1371/journal.pone.0118588
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] G-Protein-Coupled Receptors: Next Generation Therapeutic Targets in Head and Neck Cancer? (2015)
  • Author(s): Kanazawa T, Misawa K, Misawa Y, Uehara T, Fukushima H, Kusaka G, Maruta M, Carey TE
  • Journal Title: Toxins Volume: 8 Issue: 8 Pages: 2959-2984
  • DOI: 10.3390/toxins7082959
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Tumor suppressor activity and inactivation of galanin receptor type 2 by aberrant promoter methylation in head and neck cancer (2014)
  • Author(s): Misawa Y, Misawa K, Kanazawa T, Uehara T, Endo S, Mochizuki D, Yamatodani T, Carey TE, Mineta H
  • Journal Title: Cancer Volume: 120 Issue: 2 Pages: 205-213
  • DOI: 10.1002/cncr.28411
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic value of Type XXII and XXIV collagen mRNA expression in head and neck cancer patients (2014)
  • Author(s): Misawa K, Kanazawa T, Imai A, Endo S, Mochizuki D, Fukushima H, Misawa Y, Mineta H
  • Journal Title: Prognostic value of Type XXII and XXIV collagen mRNA expression in head and neck cancer patients Volume: 2 Pages: 285-291
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Prognostic value of aberrant promoter hypermethylation of tumor-related genes in early-stage head and neck cancer (2016)
  • Author(s): 三澤 清、金澤丈治、峯田周幸
  • Organizer: 第75回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] Prognostic value of aberrant promoter hypermethylation of tumor-related genes in early-stage head and neck cancer (2016)
  • Author(s): Kiyoshi Misawa, Takeharu Kanazawa, Yuki Misawa, Hiroyuki Mineta
  • Organizer: AHNS 9th International Conference 2016
  • Place of Presentation: Seatle
  • Year and Date: 2016-07-16
• [Presentation] 頭頸部癌におけるDNAメチル化解析の手法について (2016)
  • Author(s): 三澤 清、望月大極、今井篤志、岡村純、三澤由幾、金澤丈治、峯田周幸
  • Organizer: 第40回日本頭頸部癌学会
  • Place of Presentation: さいたま
  • Year and Date: 2016-06-09
• [Presentation] 頭頸部癌における神経ペプチド受容体遺伝子のプロモーター領域のメチル化について (2016)
  • Author(s): 三澤 清、望月大極、今井篤志、遠藤志織、野田和洋、石川竜司、岡村純、三澤由幾、金澤丈治、峯田周幸
  • Organizer: 第117回日本耳鼻咽喉科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-05-19
• [Presentation] Methylation analysis of neuropeptide genes in HNSCC (2015)
  • Author(s): 三澤 清、金澤丈治、峯田周幸
  • Organizer: 第74回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] 癌のエピジェネティック解析と治 (2015)
  • Author(s): 三澤 清
  • Organizer: 第76回耳鼻咽喉科臨床学会
  • Place of Presentation: 浜松
  • Year and Date: 2015-06-25
  • Invited
• [Presentation] 頭頸部癌におけるエピジェネティック分子機構について (2015)
  • Author(s): 三澤 清
  • Organizer: 第25回日本気管食道科学会認定専門医大会
  • Place of Presentation: 宇都宮
  • Year and Date: 2015-02-21 – 2015-02-22
  • Invited
• [Presentation] 中咽頭癌症例のHPV解析とDNAメチル化解析 (2014)
  • Author(s): 三澤 清、新井宏幸、石川竜司、岡村純、瀧澤義徳、三澤由幾、袴田桂、金澤丈治、峯田周幸
  • Organizer: 第38回日本頭頸部癌学会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] 頭頸部癌におけるオレキシン遺伝子の発現とプロモーター領域のメチル化との関連について (2014)
  • Author(s): 三澤 清、今井篤志、遠藤志織、望月大極、三澤由幾、高橋吾郎、金澤丈治、峯田周幸
  • Organizer: 第115回日本耳鼻咽喉科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-05-15 – 2014-05-17