Role of acrolein in the pathogenesis of diabetic retinopathy
| Project/Area Number |
26462657
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
Saito Wataru 北海道大学, 医学研究科, 客員研究員 (00339160)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
野田 航介 北海道大学, 医学研究科, 准教授 (90296666)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 糖尿病網膜症 / 不飽和アルデヒド / アクロレイン |
|---|
| Outline of Final Research Achievements |
The purpose of this study was to elucidate the involvement of acrolein, a highly reactive unsaturated aldehyde, in diabetic retinopathy (DR). We conducted immunohistochemistry for acrolein-conjugated protein, FDP-Lys, using surgically-excised tissues from patients with proliferative diabetic retinopathy (PDR) and idiopathic epiretinal membrane. In fibrovascular tissues, FDP-Lys was found in vascular components and clusters of glial cells. Morphological analysis revealed that fibrovascular tissues with FDP-Lys staining in vascular components showed high vascular density. In addition, protein level of FDP-Lys increased in the vitreous of PDR. Exposure of cultured endothelial cells to high concentration of acrolein resulted in the decrease of cell viability and proliferation, whereas lower concentration of acrolein increased cell viability and proliferation. The current data demonstrated the presence of acrolein in eyes with DR and indicate its involvement in the pathogenesis of DR.
|
Report
(4 results)
Research Products
(24 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Localization of acrolein-lys adduct in fibrovascular tissues of proliferative diabetic retinopathy2014
Author(s)
Dong Y, Noda K, Yoshida S, Tagawa Y, Ishizuka ET, Inafuku S, Kinoshita S, Murata M, Saito W, Kanda A, Ishida S
Organizer
第79回日本インターフェロン・サイトカイン学会学術集会
Place of Presentation
北海道大学フラテ会館（札幌市北区）
Year and Date
2014-06-19 – 2014-06-20
Related Report
-
-
