Signal transduction of goblet cells differentiation and clinical application

• Project/Area Number: 26462681
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nihon University (2016-2017); The University of Tokyo (2014-2015)
• Principal Investigator: YAMAGAMI Satoru 日本大学, 医学部, 教授 (10220245)
• Research Collaborator: YOKOO Seiichi
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 杯細胞 / 結膜

Outline of Final Research Achievements

We tried to investigate the stemness of isolated cells derived from human conjunctival epithelium and intra-and extracellular signal transduction system. Isolated single cells from human donor conjunctival epithelium express conjunctival epithelium-specific markers and show limited self-renewality, suggesting that isolated single cells are precursors derived from human conjunctival epithelium. FGFR is a functional receptor based on the findings of PCR, western blotting, and blocking experiments with monoclonal antibody.