Evaluation of wound healing ability in patients with diabetes based on clonal conversion of epidermal keratinocyte stem cells

• Project/Area Number: 26462739
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Keio University (2015-2017); St. Marianna University School of Medicine (2014)
• Principal Investigator: Matsuzaki Kyoichi 慶應義塾大学, 医学部(信濃町), 講師 (20278013)
• Co-Investigator(Kenkyū-buntansha): 難波 大輔 東京医科歯科大学, 難治疾患研究所, 准教授 (10380255)
• Research Collaborator: Miyamoto Akira
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 創傷治癒 / 糖尿病 / 重症下肢虚血 / 表皮角化幹細胞 / クローナル・コンバージョン / 細胞増殖 / 細胞分化 / 細胞遊走 / 表皮細胞 / 難治性潰瘍 / 細胞培養 / 感染

Outline of Final Research Achievements

Wound healing becomes prolonged in patients with diabetes. Healing is delayed even longer in patients with progressive diabetes who have developed chronic renal failure and peripheral arterial disease in the lower extremities (critical limb ischemia: CLI) as a complication of hemodialysis. This study focused on the relationship between epithelialization and the prolongation of wound healing in CLI. We evaluated cell proliferation by measuring the colony forming ability of epidermal keratinocytes from the trunks and feet of CLI patients. We also observed the migratory capacity of epidermal keratinocytes over time using a live imaging system. We found no clear difference in cell proliferation and migratory capacity between epidermal keratinocytes from CLI and from the trunk. Our findings suggest it is unlikely that epidermal keratinocyte stem cells from CLI result in significantly greater clonal conversion than trunk cells.