Roles of calcium-independent phospholipase A2 in neutrophil cytotoxicity

• Project/Area Number: 26462748
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Aiboshi Junichi 東京医科歯科大学, 医学部附属病院, 講師 (50256913)
• Co-Investigator(Kenkyū-buntansha): 小林 哲幸 お茶の水女子大学, 基幹研究院, 教授 (50178323) ; 柴田 政廣 芝浦工業大学, システム理工学部, 教授 (60158954)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: ホスホリパーゼA2 / カルシウム非依存性ホスホリパーゼA2 / 好中球 / 急性炎症 / 多臓器障害 / 出血性ショック / 血管内皮細胞 / 多臓器傷害

Outline of Final Research Achievements

Phospholipase A2 (PLA2) consists of secretory PLA2 (sPLA2), cytosolic PLA2 (cPLA2), and calcium independent PLA2 (iPLA2). Compared to sPLA2 and cPLA2, roles of the iPLA2 enzyme in acute inflammation remain ill defined. Our current studies have demonstrated that iPLA2γ may be involved in multiple neutrophil cytotoxic functions (extracellular superoxide production, degranulation, chemotaxis, adhesion, adhesion molecule surface expression) as well as the leukocyte-endothelial cell interaction in rat mesenteric microcirculation. Thus the iPLA2γ enzyme may be essential for acute inflammatory process. In addition, iPLA2γ-associated metabolites other than arachidonic acid are likely to be signaling molecules of neutrophil cytotoxicity.

Research Products

• [Journal Article] 急性炎症におけるカルシウム非依存性ホスホリパーゼA２の役割 (2016)
  • Author(s): 相星淳一、森下幸治、三上さおり、小島光暁、本藤憲一、八木雅幸、篠美和、小林哲幸、大友康裕
  • Journal Title: 日本外傷学会誌 Volume: 30 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Discrete Roles of Intracellular Phospholipase A2 in Human Neutrophil Cytotoxicity. (2015)
  • Author(s): Saori Mikami, Junichi Aiboshi, Tetsuyuki Kobayashi, Mitsuaki Kojima, Koji Mirishita, and Yasuhiro Otomo.
  • Journal Title: J. Trauma and Acute Care Surgery Volume: 79 Issue: 2 Pages: 238-246
  • DOI: 10.1097/ta.0000000000000730
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel role of group VIB Ca2+-dependent phospholipase A2γ in leukocyte-endotherial cell interaction in acute inflammation: an intravital microscopic study in rat mesentery. (2015)
  • Author(s): Mitsuaki Kojima, Junichi Aiboshi, Masahiro Shibata, Tetsuyuki Kobayashi, and Yasuhiro Otomo
  • Journal Title: J. Trauma and Acute Care Surgery Volume: 79 Issue: 5 Pages: 782-789
  • DOI: 10.1097/ta.0000000000000845
  • Peer Reviewed / Acknowledgement Compliant