A new strategy targeting microRNAs for cardiac protection against ischemia/reperfusion injury

• Project/Area Number: 26462758
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Oita University
• Principal Investigator: Teshima Yasushi 大分大学, 医学部, 講師 (10457608)
• Co-Investigator(Kenkyū-buntansha): 高橋 尚彦 大分大学, 医学部, 教授 (30263239)
• Research Collaborator: Saito Shotaro ; Nishio Satoru
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マイクロRNA / 虚血再灌流障害 / 血糖値変動 / 活性酸素種

Outline of Final Research Achievements

The present project aims to alleviate cardiac ischemia/reperfusion injury by modulating microRNAs. Reactive oxygen species (ROS) were increased by upregulating microRNA 200c and 141, and decreased by suppressing these microRNAs. We revealed that microRNA 200c and 141 increased ROS level by suppressing the activities of catalase and superoxide dismutase. We conclude that modulating these microRNAs may be a possible target against ischemia/reperfusion injury in the heart.

Research Products

• [Journal Article] Role of atrial endothelial cells in the development of atrial fibrosis and fibrillation in response to pressure overload (2017)
  • Author(s): Kume O, Teshima Y, Abe I, Ikebe Y, Oniki T, Kondo H, Saito S, Fukui A, Yufu K, Miura M, Shimada T, Takahashi N.
  • Journal Title: Cariovasc Pathology Volume: 27 Pages: 18-25
  • DOI: 10.1016/j.carpath.2016.12.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hyperleptinemia Exacerbates High-Fat Diet-Mediated Atrial Fibrosis and Fibrillation. (2017)
  • Author(s): Fukui A, Ikebe-Ebata Y, Kondo H, Saito S, Aoki K, Fukunaga N, Shinohara T, Masaki T, Teshima Y, Takahashi N.
  • Journal Title: J Cardiovasc Electrophysiol. Volume: 印刷中 Issue: 6 Pages: 702-710
  • DOI: 10.1111/jce.13200
  • Peer Reviewed / Open Access
• [Journal Article] Mast Cells Play an Important Role in the Pathogenesis of Hyperglycemia-Induced Atrial Fibrillation (2016)
  • Author(s): Uemura K, Kondo H, Ishii Y, Kobukata M, Haraguchi M, Imamura T, Otsubo T, Ikebe-Ebata Y, Abe I, Ayabe R, Saito S, Aoki K, Nagano-Torigoe Y, Akioka H, Shinohara T, Teshima Y, Masaki T, Yufu K, Nakagawa M, Takahashi N
  • Journal Title: J Cardiovasc Electrophysiol Volume: - Issue: 8 Pages: 981-989
  • DOI: 10.1111/jce.12995
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Glucose Fluctuations Aggravate Cardiac Susceptibility to Ischemia/Reperfusion Injury by Modulating MicroRNAs Expression (2016)
  • Author(s): Saito S, Thuc LC, Teshima Y, Nakada C, Nishio S, Kondo H, Fukui A, Abe I, Ebata Y, Saikawa T, Moriyama M, Takahashi N.
  • Journal Title: Circulation Journal Volume: 80 Issue: 1 Pages: 186-195
  • DOI: 10.1253/circj.CJ-14-1218
  • NAID: 130005116977
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Role of Indoxyl Sulfate as a Predisposing Factor for Atrial Fibrillation in Renal Dysfunction. (2015)
  • Author(s): Aoki K, Teshima Y, Kondo H, Saito S, Fukui A, Fukunaga N, Nawata T, Shimada T, Takahashi N, Shibata H.
  • Journal Title: Journal of American Heart Association Volume: 4 Issue: 10
  • DOI: 10.1161/jaha.115.002023
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Glucose fluctuations increase the incidence of atrial fibrillation in diabetic rats (2014)
  • Author(s): Saito S, Teshima Y, Fukui A, Kondo H, Nishio S, Nakagawa M, Saikawa T, Takahashi N.
  • Journal Title: Cardiovascular Research Volume: 104 Issue: 1 Pages: 5-14
  • DOI: 10.1093/cvr/cvu176
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Microstructural Features of Atrial Appendage in Patients with Atrial Fibrillation (2017)
  • Author(s): Sonoda Y, Teshima Y, Ikebe Y, Abe I, Yufu K, Shimada T, Miyamoto S, Takahashi N
  • Organizer: 第81回日本循環器学会総会学術集会
  • Place of Presentation: ANAクラウンプラザホテル金沢（石川県・金沢市）
  • Year and Date: 2017-03-17
• [Presentation] Glucose Fluctuations Impair Cardiac Sinus Node Function in Diabetic Animal Models (2017)
  • Author(s): Ikebe Y, Teshima Y, Sonoda Y, Abe I, Yufu K, Shimada T, Miyamoto S, Takahashi N
  • Organizer: 第81回日本循環器学会総会学術集会
  • Place of Presentation: ANAクラウンプラザホテル金沢（石川県・金沢市）
  • Year and Date: 2017-03-17
• [Presentation] Glucose Fluctuations by Repeated Hypoglycemic Episodes Facilitate Atrial Remodeling and Atrial Fibrillation (2016)
  • Author(s): Teshima Y, Saito S, Ikebe Y, Abe I, Kondo K, Yufu K, Takahashi N
  • Organizer: 第80回日本循環器学会総会学術集会
  • Place of Presentation: 仙台国際センター（仙台市）
  • Year and Date: 2016-03-22
• [Presentation] 糖尿病患者における血糖変動と心臓自律神経機能 (2015)
  • Author(s): 手嶋泰之、長野徳子、中川幹子、高橋尚彦
  • Organizer: 第62回日本臨床検査医学会学術集会
  • Place of Presentation: 長良川国際会議場（岐阜市）
  • Year and Date: 2015-11-19
• [Presentation] 糖尿病における血糖変動は心房細動誘発性を亢進する (2015)
  • Author(s): 手嶋泰之、斉藤聖多郎、池邉有希、安部一太郎、近藤秀和、高橋尚彦
  • Organizer: 第55回日本臨床化学会学術集会
  • Place of Presentation: 大阪大学（大阪府、吹田市）
  • Year and Date: 2015-10-30
• [Presentation] Glucose fluctuations increase inducibility of atrial fibrillation in diabetic rats (2015)
  • Author(s): 斉藤聖太郎
  • Organizer: 第79回日本循環器学会年次学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2015-04-24 – 2015-04-26
• [Presentation] Glucose Fluctuations Aggravate Myocardial Ischemia/Reperfusion Injury in Type 2 Diabetic Rats (2015)
  • Author(s): Saito S, Teshima Y, Abe I, Ikebe Y, Kondo K, Nishio S, Yufu K, Takahashi N
  • Organizer: 第79回日本循環器学会総会学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2015-04-24