Can opioid exacerbate acute lung injury via TLR4?

• Project/Area Number: 26462760
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Matsuyama Hiroki 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (80515289)
• Co-Investigator(Kenkyū-buntansha): 天谷 文昌 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60347466)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: オピオイド / acute lung injury / 免疫細胞 / ARDS / 肺損傷 / 人工呼吸関連肺損傷 / 急性呼吸窮迫症候群

Outline of Final Research Achievements

We investigated the effect of remifentanil on the inflammatory reaction in the lung during acute lung injury.
Remifentanil was continuously infused to rats. Remifentanil dose was determined based on the behavioral assessment of withdrawal hyperalgesia. Expression of inflammatory cytokines in the lung of rats treated with LPS with or without remifentanil was measured by real time PCR. Opioid receoptor and TLR5 expression in the lung was investigated by immunohistochemistry.
LPS induced induction of inflammatory cytokine in the lung. The effect of remifentanil on the LPS induced induction of inflammatory cytokine was not clear. We detected cells positive for opioid receptors or TLR4 in the lung tissue.

Research Products

• [Journal Article] Risk factors and prognosis of pain events during mechanical ventilation: a retrospective study (2017)
  • Author(s): Yamashita A, Yamasaki M, Matsuyama H, Amaya F
  • Journal Title: J Intensive Care Volume: 5 Issue: 1 Pages: 17-17
  • DOI: 10.1186/s40560-017-0212-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] mTOR signaling controls VGLUT2 expression to maintain pain hypersensitivity after tissue injury. (2015)
  • Author(s): Izumi Y, Sasaki M, Hashimoto S, Sawa T, Amaya F.
  • Journal Title: Neuroscience. Volume: 308 Pages: 169-179
  • DOI: 10.1016/j.neuroscience.2015.09.013
  • Peer Reviewed / Open Access
• [Journal Article] Risk Factors for Poor Prognosis after Emergency Surgery for Acute Abdomen (2015)
  • Author(s): Maeda C, Matsuoka Y, Shimosato G, Hirata M, Amaya F
  • Journal Title: Masui Volume: 64 Pages: 591-596
  • Peer Reviewed