| Project/Area Number |
26462774
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
AKIYAMA Tsuyoshi 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (70202554)
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| Co-Investigator(Renkei-kenkyūsha) |
INAGAKI Tadakatsu 独立行政法人国立循環器病研究センター, 血管生理学部, 上級研究員 (20638366)
SHIMIZU Shuji 独立行政法人国立循環器病研究センター, 循環動態制御部, 上級研究員 (80443498)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 心臓 / 虚血・再灌流 / モノアミン / ヒドロキシラジカル / マイクロダイアリシス法 / モノアミンオキシダーゼ / 心虚血・再灌流 / ミオグロビン |
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| Outline of Final Research Achievements |
We applied microdialysis technique to the heart of anesthetized rats. Dialysate samples were collected during 30 min of induced ischemia followed by 60 min of reperfusion. We monitored dialysate 3,4-dihydrobenzoic acid (3,4-DHBA) concentration as an index of hydroxyl radical production using a trapping agent (4-hydroxybenzoic acid), and dialysate myoglobin concentration as an index of cardiomyocyte injury in the ischemic region. Dialysate 3,4-DHBA concentration increased during ischemia and further increased after reperfusion. Pargyline, a MAO inhibitor, suppressed the averaged increase in dialysate 3,4-DHBA concentration during ischemia and reperfusion. Dialysate myoglobin concentration increased during ischemia and further increased after reperfusion. Pargyline reduced the averaged dialysate myoglobin concentration during ischemia and reperfusion. MAO plays a significant role in hydroxyl radical production and cardiomyocyte injury during ischemia and after reperfusion.
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