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Study on the pathogenicity of dental biofilm by molecular genetic technics

Research Project

Project/Area Number 26462801
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

Hamada Nobushiro  神奈川歯科大学, 大学院歯学研究科, 教授 (20247315)

Co-Investigator(Kenkyū-buntansha) 高橋 俊介  神奈川歯科大学, 大学院歯学研究科, 准教授 (60206810)
渡辺 清子  神奈川歯科大学, 大学院歯学研究科, 講師 (70148021)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsグラム陽性桿菌 / Actinomyces naeslundii / バイオフィルム / 歯周炎 / 通性嫌気性グラム陽性桿菌 / 共凝集 / 歯槽骨吸収 / サイトカイン / 破骨細胞
Outline of Final Research Achievements

A. naeslundii peptidoglycan induces the production of inflammatory cytokines and activates osteoclasts in alveolar bone resorption. Expressions of IL-1β, IL-6, and TNF-α mRNA were apparent in BALB/c mouse peritoneal macrophages stimulated with A. naeslundii PGN. In experimental periodontitis, the oral challenge of A. naeslundii resulted in the induction of alveolar bone loss in rats. These results suggest that peptidoglycan of A. naeslundii is an important virulence factor in the development of periodontitis. We could not succeed in the mutation of A. naeslundii fimbriae, however titanium oxide photocatalytic properties contribute to the reduction of bacterial biofilms and aid in the prevention of periodontal diseases.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Porphyromonas gingivalis infection modifies oral microcirculation and aortic vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP).2016

    • Author(s)
      Funaki S, Tokutomi F, Wada-Takahashi S, Yoshino F, Yoshida A, Maehata Y, Miyamoto C, Toyama T, Sato T, Hamada N, Lee M-C, Takahashi S-S
    • Journal Title

      Microbial Pathogenesis

      Volume: 92 Pages: 36-42

    • DOI

      10.1016/j.micpath.2015.12.009

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Protective effects of (6R)-5,6,7,8-tetrahydro-L-biopterin on local ischemia/reperfusion-induced suppression of reactive hyperemia in rat gingiva2016

    • Author(s)
      Tanaka Y, Toyama T, Wada-Takahashi S, Sasaki H, Miyamoto C, Maehata Y, Yoshino F, Yoshida A, Takahashi S-S, Watanabe K, Lee M-C, Todoki K, Hamada N
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 58 Issue: 1 Pages: 69-75

    • DOI

      10.3164/jcbn.15-69

    • NAID

      130005116629

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficacy of a solar-powered TiO2 semiconductor electric toothbrush on P. gingivalis biofilm2015

    • Author(s)
      Sato T, Hirai N, Oishi Y, Uswak G, Komiyama K, Hamada N
    • Journal Title

      American Journal of Dentistry

      Volume: 28(2) Pages: 81-84

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Efficacy of a solar-powered TiO2 semiconductor electric toothbrush on P. gingivalis biofilm.2015

    • Author(s)
      Sato T, Hirai N, Oishi Y, Uswak G, Komiyama K, Hamada N
    • Journal Title

      Am J Dent

      Volume: 28 Pages: 81-84

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Porphyromonas gingivalis-induced alveolar bone loss is accelerated in the stroke-prone spontaneously hypertensive rat.2015

    • Author(s)
      Tokutomi F, Wada-Takahashi S, Sugiyama S, Toyama T, Sato T, Hamada N, Tsukinoki K, Takahashi SS, Lee MC
    • Journal Title

      Arch Oral Biol

      Volume: 60 Issue: 6 Pages: 911-918

    • DOI

      10.1016/j.archoralbio.2015.02.012

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ameliorating effects of Juzentaihoto on restraint stress and P. gingivalis-induced alveolar bone loss.2014

    • Author(s)
      Takeda O, Toyama T, Watanabe K, Sato T, Sasaguri K, Akimoto S, Sato S, Kawata T, Hamada N
    • Journal Title

      Arch Oral Bio

      Volume: 59 Issue: 11 Pages: 1130-1138

    • DOI

      10.1016/j.archoralbio.2014.06.010

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Pycnogenol chewing gum reduced plaque formation and oral malodor2015

    • Author(s)
      Hamada N, Watanabe K
    • Organizer
      Asian Pacific Society of Periodontology Meeting
    • Place of Presentation
      Bali
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Efficacy of a toothpaste containing natural ingredient against oral bacteria.2015

    • Author(s)
      Sato T, Hamada N
    • Organizer
      The 93rd General Session and Exhibition of the IADR
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Efficacy of a solar-powered TiO2 semiconductor electric toothbrush.2014

    • Author(s)
      Sato T, Oishi Y, Hamada N
    • Organizer
      The 7th World Congress on Preventive & Regenerative Medicine
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2014-11-04 – 2014-11-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯磨剤の抗菌効果および歯槽骨吸収抑制効果の検討.2014

    • Author(s)
      佐藤武則,早坂奈美,遠山歳三,高橋俊介,浜田信城
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Antimicrobial activity and inhibitory effect of alveolar bone resorption by a toothpaste containing Pycnogenol®.2014

    • Author(s)
      Sato T, Toyama T, Takahashi S-S, Hamada N
    • Organizer
      International Union of Microbiological Societies
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2014-07-26 – 2014-08-01
    • Related Report
      2014 Research-status Report
  • [Presentation] 太陽電池内臓電動歯ブラシの酸化チタン光触媒作用によるバイオフィルム除去効果.2014

    • Author(s)
      佐藤武則,渡辺清子,浜田信城
    • Organizer
      医学生物学電子顕微鏡技術学会第30回学術講演会および総会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-23
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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