| Project/Area Number |
26462823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
石崎 明 岩手医科大学, 歯学部, 教授 (20356439)
衣斐 美歩 岩手医科大学, 薬学部, 助教 (30609665)
三上 俊成 岩手医科大学, 歯学部, 准教授 (40405783)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTSUKA Masato 東海大学, 医学部, 講師 (90372945)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 癌細胞 / ニッチ / マクロファージ / 免疫抑制 / 接着 / マクロファージコロニー刺激因子 / M2-マクロファージ / 骨髄由来細胞 / 間葉系幹細胞 / 共培養 / 接着因子 / 口腔癌 / フィブロサイト / M2マクロファージ / 創傷治癒 / 口腔癌細胞 / MSC / CCR2 / ホーミング |
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| Outline of Final Research Achievements |
Cell-cell interactions among the cells consisting cancer cell niche was investigated. Bone marrow-derived lineage-positive (Lin+) blood cells proliferated and differentiated into Immunosuppressive/anti-inflammatory macrophage (Mφ), M2-Mφs, by cooperation with the bone marrow-derived mesenchymal stem cells (MSCs) under hypoxic condition: MSCs not only promoted proliferation of undifferentiated M2-Mφs, pre-M2-Mφs, in the Lin+ fraction via a proliferative effect of the MSCs-secreted macrophage colony-stimulating factor, but also promoted M2-Mφ polarization of the pre-M2-Mφs through cell-to-cell contact with the pre-M2-Mφs. The inhibitory experiments using the neutralizing antibodies and the adhesion molecule inhibitors suggested that the cell-to-cell adhesion through LFA-1 on pre-M2-Mφs and ICAM-1 on MSCs was supposed to promoted the M2-Mφ polarization. Thus, in the cancer niche, the induced M2-Mφs seemed to implicate in the progressive transformation of the oral cancer cells.
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