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Regulation of bone remodeling by Annexin A5 at tendon/ligament insertion sites

Research Project

Project/Area Number 26462825
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionTsurumi University

Principal Investigator

Shimada Akemi  鶴見大学, 歯学部, 講師 (00339813)

Co-Investigator(Kenkyū-buntansha) 二藤 彰  鶴見大学, 歯学部, 教授 (00240747)
和田 悟史  鶴見大学, 歯学部, 助教 (20581119)
山下 照仁  松本歯科大学, 総合歯科医学研究所, 准教授 (90302893)
Research Collaborator ARAI Yoshinori  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsAnnexin a5 / 腱 / 靭帯 / 骨 / アネキシンA5
Outline of Final Research Achievements

Entheses can be further divided to fibrocartilaginous and fibrous entheses according to the type of tissue present at the skeletal attachment site. In Anxa5-deficient (Anxa5-/-) mice, the sizes of bone ridge outgrowths at the fibrocartilaginous entheses of tibiae and femur were increased after 7 weeks of age. This bone overgrowth was not observed at the fibrous enthesis where Anxa5-lacZ is hardly expressed. At the fibrocartilaginous entheses, more ALP-expressing cells were observed in the fibrocartilage layer outside of the bone in the mutant mice. Then, to examine the role of Anxa5 on cartilage differentiation, we performed Anxa5 gene knockdown in vitro using siRNA. Depletion of endogenous Anxa5 with siRNA increased mRNA expressions of differentiated marker of chondrocyte. Together, these results suggest that Anxa5 prevents bone overgrowth at the fibrocartilaginous entheses.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (42 results)

All 2017 2016 2015 2014

All Journal Article (14 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 14 results,  Open Access: 12 results,  Acknowledgement Compliant: 2 results) Presentation (28 results)

  • [Journal Article] Platypus and opossum calcitonins exhibit strong activities, even though they belong to mammals.2017

    • Author(s)
      Yamashita T, Udagawa N, Thirukonda GJ, Uehara S, Yamauchi H, Suzuki N, Li F, Kobayashi Y, Takahashi N.
    • Journal Title

      Gen Comp Endocrinol.

      Volume: 246 Pages: 270-278

    • DOI

      10.1016/j.ygcen.2017.01.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cationized gelatin hydrogels mixed with plasmid DNA induce stronger and more sustained gene expression than atelocollagen at calvarial bone defects in vivo.2016

    • Author(s)
      Komatsu K, Shibata T, Shimada A, Ideno H, Nakashima K, Tabata Y, Nifuji A.
    • Journal Title

      J Biomater Sci Polym Ed.

      Volume: 27 Issue: 5 Pages: 419-430

    • DOI

      10.1080/09205063.2016.1139486

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The dynamin inhibitor dynasore inhibits bone resorption by rapidly disrupting actin rings of osteoclasts.2016

    • Author(s)
      Thirukonda GJ, Uehara S, Nakayama T, Yamashita T, Nakamura Y, Mizoguchi T, Takahashi N, Yagami K, Udagawa N, Kobayashi Y.
    • Journal Title

      J Bone Miner Metab

      Volume: 34 Issue: 4 Pages: 395-405

    • DOI

      10.1007/s00774-015-0683-1

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular regulatory mechanisms of osteoclastogenesis through cytoprotective enzymes2016

    • Author(s)
      Kanzaki, H. Shinohara, F. Kanako, I. Yamaguchi, Y. Fukaya, S. Miyamoto, Y. Wada, S. Nakamura, Y.
    • Journal Title

      Redox Biol

      Volume: 8 Pages: 186-191

    • DOI

      10.1016/j.redox.2016.01.006

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bardet-Biedl Syndrome 3 regulates development of cranial base midline structures.2016

    • Author(s)
      Kawasaki M, Izu Y, Hayata T, Ideno H, Nifuji A, Sheffield VC, Ezura Y, Noda M.
    • Journal Title

      Bone

      Volume: 89 Pages: 419-430

    • DOI

      10.1016/j.bone.2016.02.017

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-performance scaffolds on titanium surfaces: Osteoblast differentiation and mineralization promoted by a globular fibrinogen layer through cell-autonomous BMP signaling.2015

    • Author(s)
      Horasawa N, Yamashita T, Uehara S, Udagawa N
    • Journal Title

      Mater Sci Eng C Mater Biol Appl

      Volume: 46 Pages: 86-96

    • DOI

      10.1016/j.msec.2014.10.025

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Wnt16 regulates osteoclast differentiation in conjunction with Wnt5a.2015

    • Author(s)
      Kobayashi Y, Thirukonda GJ, Nakamura Y, Koide M, Yamashita T, Uehara S, Kato H, Udagawa N, Takahashi N
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 463 Issue: 4 Pages: 1278-1283

    • DOI

      10.1016/j.bbrc.2015.06.102

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Roles of the histone methyltransferase G9a in the development and differentiation of mesenchymal tissues.2015

    • Author(s)
      Ideno H, Nakashima K, Nifuji A.
    • Journal Title

      J Phys Fitness Sports Med

      Volume: 4 Pages: 357-362

    • NAID

      130005113195

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization.2015

    • Author(s)
      Kanemoto S, Kobayashi Y, Yamashita T, Miyamoto T, Cui M, Asada R, Cui X, Hino K, Kaneko M, Takai T, Matsuhisa K, Takahashi N, Imaizumi K.
    • Journal Title

      J Cell Sci

      Volume: 128 Pages: 4353-4365

    • DOI

      10.1242/jcs.176057

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro2015

    • Author(s)
      Wada S, Ideno H, Shimada A, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Shinkai Y, Tachibana M, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: in press Issue: 1 Pages: 13-20

    • DOI

      10.1007/s00418-015-1318-2

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Coordinated expression of H3K9 histone methyltransferases during tooth development in mice2015

    • Author(s)
      Kamiunten T, Ideno H, Shimada A, Nakamura Y, Kimura H, Nakashima K, Nifuji A.
    • Journal Title

      Histochem Cell Biol.

      Volume: 143 Issue: 3 Pages: 259-266

    • DOI

      10.1007/s00418-014-1284-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient expansion of mouse primary tenocytes using a novel collagen gel culture method2014

    • Author(s)
      Akemi Shimada, Satoshi Wada, Kouji Inoue, Hisashi Ideno, Taichi Kamiunten, Koichiro Komatsu, Akira Kudo, Yoshiki Nakamura,et al
    • Journal Title

      Histochem Cell Biol

      Volume: (in press) Issue: 2 Pages: 205-215

    • DOI

      10.1007/s00418-014-1191-4

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Noncanonical Wnt5a enhances Wnt/-catenin signaling during osteogenesis.2014

    • Author(s)
      Okamoto, M., Udagawa, N., Yamashita, T., Nakamichi, Y., Uehara, S., Kato, H., Saito, N., Minami, Y., Takahashi, N., Kobayashi, Y.
    • Journal Title

      Sci. report

      Volume: 4 Issue: 1 Pages: 4493-4493

    • DOI

      10.1038/srep04493

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arctigenin inhibits osteoclast differentiation and function by suppressing both calcineurin-dependent and osteoblastic cell-dependent NFATc1 pathways.2014

    • Author(s)
      Yamashita T, Uehara S, Udagawa N, Li F, Kadota S, Esumi H, Kobayashi Y, Takahashi N
    • Journal Title

      PLoS One

      Volume: 9 Issue: 1 Pages: 85878-85878

    • DOI

      10.1371/journal.pone.0085878

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒストンメチル化酵素G9aのマウス歯牙発生における機能2016

    • Author(s)
      上運天太一、出野尚、島田明美、中村芳樹、木村宏、新井嘉則、立花誠、中島和久、二藤彰
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒストンメチル化酵素G9aの骨芽細胞分化における機能2016

    • Author(s)
      出野尚、小松浩一郎、島田明美、新井嘉則、中島和久、荒木良子、安倍真澄、立花誠、木村宏、二藤彰
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 腱組織形成におけるヒストンメチル化酵素G9aの機能2016

    • Author(s)
      和田悟史、出野尚、島田明美、上運天太一、中村芳樹、中島和久、木村宏、眞貝洋一、立花誠、二藤彰
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Annexin a5による腱・靭帯付着部(enthesis)における骨増大の抑制2016

    • Author(s)
      島田明美、新井嘉則、和田悟史、出野尚、中島和久、小松浩一郎、山下照仁、江面陽一、中村芳樹、二藤彰
    • Organizer
      第2回日本アネキシン研究会年会
    • Place of Presentation
      札幌医科大学(北海道、札幌市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Annexin a5による腱・靭帯付着部(enthesis)における線維軟骨層の石灰化の制御2016

    • Author(s)
      島田明美、新井嘉則、和田悟史、出野尚、中島和久、小松浩一郎、山下照仁、江面陽一、網塚憲生、中村芳樹、二藤彰
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府、大阪市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス歯牙形成過程におけるヒストンメチル化酵素G9aの機能2016

    • Author(s)
      上運天太一、出野尚、島田明美、中村芳樹、中島和久、木村宏、立花誠、二藤彰
    • Organizer
      第34回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府、大阪市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Histone methyltransferase G9a is essential to progress osteoblastic differentiation in vitro, and skull bone formation in vivo.2015

    • Author(s)
      Ideno H, Komatsu K, Shimada A, Kamiunten T, Arai Y, Nakashima K, Araki R, Nakamura Y, Abe M, Tachibana M, Kimura H, Nifuji A.
    • Organizer
      ASCB annual meeting
    • Place of Presentation
      San Diego Convention Center (USA, CA, San Diego)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Essential roles of H3K9MTase G9a during tooth development.2015

    • Author(s)
      Kamiunten T, Shimada A, Ideno H, Nakamura Y, Kimura H, Tachibana M, Nakashima K, Nifuji A.
    • Organizer
      ASCB annual meeting
    • Place of Presentation
      San Diego Convention Center (USA, CA, San Diego)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9a遺伝子欠損による腱の形成阻害2015

    • Author(s)
      和田悟史、出野尚、島田明美、上運天太一、中村芳樹、中島和久、木村宏、眞貝洋一、立花誠、二藤彰
    • Organizer
      第38回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県、神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Deletion of G9a, histone methyltransferase, causes impaired bone formation.2015

    • Author(s)
      Ideno H, Komatsu K, Shimada A, Kamiunten T, Arai Y, Nakashima K, Araki R, Nakamura Y, Abe M, Tachibana M, Kimura H, Nifuji A
    • Organizer
      Joint ANZBMS, MEPSA and MBSANZ Annual Scientific Meeting
    • Place of Presentation
      Hotel Grand Chancellor Hobart (Australia, Tasmania)
    • Year and Date
      2015-11-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Annexin A5 inhibits bony outgrowth at tendon/ligament insertion sites.2015

    • Author(s)
      Shimada A, Arai Y, Wada S, Ideno H, Kamiunten T, Nakashima K, Komatsu K, Yamashita T, Ezura Y, Amizuka N, Pöschl E, Brachvogel B, Nakamura Y, Nifuji A
    • Organizer
      ASBMR 2015 annual meeting
    • Place of Presentation
      Washington State Convention Center (USA, WA, Seattle)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
  • [Presentation] Histone 3 lysine 9 methyltransferase G9a is essential for the growth and differentiation of tenocytes.2015

    • Author(s)
      Wada S, Ideno H, Shimada H, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Tachibana M, Nifuji A.
    • Organizer
      ASBMR 2015 annual meeting
    • Place of Presentation
      Washington State Convention Center (USA, WA, Seattle)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
  • [Presentation] アネキシンA5欠損マウスは歯の咬耗と腱付着部における顎骨の肥大を呈する2015

    • Author(s)
      島田明美, 小松浩一郎, 新井嘉則, 大貫芳樹, 中島和久, 山下照仁, 奥村敏, 二藤彰
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県、新潟市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストン3リジン9メチル化酵素G9aは正常な歯牙発生に必要である2015

    • Author(s)
      上運天太一, 出野尚, 中村芳樹, 中島和久, 島田明美, 二藤彰
    • Organizer
      第57回歯科基礎医学会学術大会
    • Place of Presentation
      朱鷺メッセ(新潟県、新潟市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯周病モデルにおける好中球エラスターゼ阻害剤の歯周組織に与える影響2015

    • Author(s)
      氏家優子、島田明美、小松浩一郎、大島朋子、二藤彰、大井田新一郎、五味一博
    • Organizer
      第58回秋季日本歯周病学会学術大会
    • Place of Presentation
      アクトシティ浜松(静岡県、浜松市)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] nnexin a5による腱・靭帯付着部(enthesis)における骨形成の調節2015

    • Author(s)
      島田明美、新井嘉則、和田悟史、出野尚、上運天太一、中島和久、小松浩一郎、山下照仁、江面陽一、網塚憲生、中村芳樹、二藤彰
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都、新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9aは腱細胞の増殖および分化に必須である2015

    • Author(s)
      和田悟史、出野 尚、島田明美、上運天太一、中村芳樹、中島和久、木村宏、立花誠、二藤 彰
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都、新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9a欠損マウスは骨形成阻害を示す2015

    • Author(s)
      出野 尚, 小松浩一郎, 島田明美, 上運天太一、新井嘉則、中島和久、荒木良子, 中村芳樹, 安倍真澄、立花 誠、木村 宏、二藤 彰
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都、新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9a遺伝子の歯胚間葉における欠損は歯の成長阻害を生ずる2015

    • Author(s)
      上運天太一、出野尚、島田明美、中島和久、中村芳樹、木村宏、立花誠、二藤彰
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都、新宿区)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Annexin A5欠損マウスは咀嚼筋肥大、歯の咬耗、口腔顔面の骨肥大を呈する2014

    • Author(s)
      島田明美、和田悟史、出野尚、上運天太一、小松浩一郎、工藤明、中村芳樹、中島和久、二藤彰
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜パシフィコ(神奈川県横浜市)
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯の発生分化におけるヒストンメチル化酵素の発現と機能2014

    • Author(s)
      上運天太一、出野尚、島田明美、中島和久、中村芳樹、二藤彰
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜パシフィコ(神奈川県横浜市)
    • Year and Date
      2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9aによる間葉系細胞の増殖と分化の調節2014

    • Author(s)
      出野尚、島田明美、上運天太一、和田悟史、小松浩一郎、中村芳樹、安倍真澄、中島和久、二藤彰
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 腱細胞の発生分化におけるヒストンメチル化酵素G9aの機能2014

    • Author(s)
      和田悟史、出野尚、島田明美、上運天太一、中島和久、中村芳樹、二藤彰
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯の発生分化におけるヒストンメチル化酵素の発現と機能2014

    • Author(s)
      上運天太一、出野尚、島田明美、中島和久、中村芳樹、二藤彰
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 効率的で安定的なマウス腱細胞の初代培養法2014

    • Author(s)
      島田明美、和田悟史、出野尚、上運天太一、小松浩一郎、工藤明、中村芳樹、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9aによる腱細胞の分化制御2014

    • Author(s)
      和田悟史、島田明美、出野尚、立花誠、中村芳樹、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-26
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒストン3リジン9メチル化酵素群(G9a, Glp, Suv39h1, Prdm2)のマウス歯牙発生における発現と機能2014

    • Author(s)
      上運天太一、島田明美、出野尚、中村芳樹、木村宏、立花誠、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 間葉系細胞の増殖・分化過程におけるヒストンメチル化酵素G9aの機能2014

    • Author(s)
      出野尚、島田明美、上運天太一、和田悟史、小松浩一郎、荒木良子、立花誠、中村芳樹、安倍真澄、中島和久、二藤彰
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-24
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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