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KLF-dependent cell differentiation- and EMT-inducing factor and suppression of oral carcinoma progression

Research Project

Project/Area Number 26462859
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionThe Nippon Dental University

Principal Investigator

Imai Kazushi  日本歯科大学, 生命歯学部, 教授 (10328859)

Co-Investigator(Kenkyū-buntansha) 須藤 遙  日本歯科大学, 生命歯学部, 講師 (20372980)
千葉 忠成  日本歯科大学, 生命歯学部, 准教授 (60350138)
Co-Investigator(Renkei-kenkyūsha) MIHARA Nozomi  日本歯科大学, 生命歯学部, 助教 (00803264)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords口腔癌 / KLF / プロモーター / KLF5 / 口腔癌細胞 / サイレンサー / Sp3 / 転写因子 / Sp1
Outline of Final Research Achievements

Kruppel-like factor (KLF) plays a critical role in epithelial tissue development and homeostasis. Expression balance of KLF4 and KLF5 is considered to regulate carcinoma cell phenotypes and carcinoma progression. However, very little is known about a role KLF4/5 in oral carcinomas.
This study demonstrated that KLF4/5 regulate differentiation and dedifferentiation of oral carcinoma cells and that expression of KLF4 are suppressed by hypermethylation of the gene promoter. In regard to KLF5 expression, a region from +145 to +330 act as a minimal essential region and Sp3 binding to GC box was a indispensable event.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(9 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 3 results) Presentation (5 results)

  • [Journal Article] MALT1 Inhibition of Oral Carcinoma Cell Invasion and ERK/MAPK Activation.2017

    • Author(s)
      Chiba T, Soeno Y, Shirako Y, Sudo H, Yagishita H, Taya Y, Kawashiri S, Okada Y, Imai K.
    • Journal Title

      Gene

      Volume: 95 Pages: 36-43

    • DOI

      10.1177/0022034515621740

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Krüppel-like factors 4 and 5 expression and their involvement in differentiation of oral carcinomas2015

    • Author(s)
      Shibata M, Chiba T, Matsuoka T, Mihara N, Kawashiri S, Imai K
    • Journal Title

      Int. J. Clin. Exp. Pathol.

      Volume: 8 Pages: 3701-3709

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Krüppel-like factor 4 expression in oral carcinoma cells and hypermethylation at the gene promoter2015

    • Author(s)
      Yamaguchi A, Kuroyama K, Tokura A, Saito A, Arikawa H, Hasebe T, Usui D, Yamaguchi K, Chiba T, Imai K
    • Journal Title

      BMC Oral Health

      Volume: 16 Pages: 13-13

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kruppel-like factors 4 and 5 expression and their involvement in differentiation of oral carcinomas.2015

    • Author(s)
      Shibata M, Chiba T, Matsuoka T, Mihara N, Kawashiri S, Imai K.
    • Journal Title

      Int J Clin Exp Pathol

      Volume: 8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Kruppel-like factor 5遺伝子発現の必要最小領域とSp3の関与2016

    • Author(s)
      美原希美
    • Organizer
      第58回日本歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター(北海道、札幌市)
    • Year and Date
      2016-08-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 口腔癌細胞におけるKruppel-like factor 4発現とプロモーターのメチル化2015

    • Author(s)
      山口光祐,千葉忠成,美原希美,須藤遥,今井一志
    • Organizer
      第57回日本歯科基礎医学会学術大会・総会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Promoter analysis of Kruppel-like factor 5 gene.2015

    • Author(s)
      Mihara N, Chiba T, Sudo H, Imai K
    • Organizer
      General Session and Exhibition of the IADR
    • Place of Presentation
      ボストン(米国)
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Kruppel-like factor 5遺伝子のプロモーター解析2014

    • Author(s)
      美原希美、千葉忠成、須藤遥、今井一志
    • Organizer
      歯科基礎医学会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 口腔癌におけるKLF4/5の発現と上皮ー間葉移行への関与2014

    • Author(s)
      柴田征紀、川尻秀一、今井一志
    • Organizer
      日本口腔科学会
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2014-05-08
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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