Evaluation of animal-free systems in human deatal pulp cells using a spinal cord injury model
| Project/Area Number |
26463001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
飯田 一規 岐阜大学, 大学院医学系研究科, 助教 (30585237)
玉置 也剛 岐阜大学, 大学院医学系研究科, 助教 (40585303)
柴田 敏之 岐阜大学, 大学院医学系研究科, 教授 (50226172)
畠山 大二郎 岐阜大学, 医学部附属病院, 講師 (60377653)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 再生歯学 / 歯髄由来細胞 / iPS細胞 |
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| Outline of Final Research Achievements |
Human dental pulp cells (hDPC) are a promising resource for regenerative medicine and tissue engineering. However, current protocols use reagents of animal origin (mainly fetal bovine serum) that carry the potential risk of infectious diseases and unwanted immunogenicity. Here, we report a chemically defined protocol to isolate and maintain the growth and differentiation potential of hDPCs. The chemically defined culture conditions can be used for the safe establishment of iPSCs and will find utility in applications for cell-based regenerative medicine.
The central nervous system in adult mammals cannot be repaired spontaneously after spinal cord injury (SCI). However, SCI treatment has improved in recent years following the development of cell transplantation therapy. We recently found that basic fibroblast growth factor (FGF2)-pre-treated hDPCs cultured under chemically defined conditions can improve recovery in rat SCI models.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Derivation of iPSCs after culture of human dental pulp cells under defined conditions2014
Author(s)
Takeda-Kawaguchi T, Sugiyama K, Chikusa S, Iida K, Aoki H, Tamaoki N, Hatakeyama D, Kunisada T, Shibata T, Fusaki N, Tezuka K.
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Journal Title
PLoS One. 2014 Dec 18;9(12):e115392
Volume: 18
Issue: 12
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The homeobox gene DLX4 promotes generation of human induced pluripotent stem cells2014
Author(s)
Naritaka Tamaoki, Kazutoshi Takahashi, Hitomi Aoki, Kazuki Iida, Tomoko Kawaguchi, Daijirou Hatakeyama, Masatoshi Inden, Naoyuki Chosa, Akira Ishisaki, Takahiro Kunisada, Toshiyuki Shibata, Naoki Goshima, Shinya Yamanaka, and Ken-ichi Tezuka
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Journal Title
Scientific Reports
Volume: なし
Issue: 1
Pages: 7283-7283
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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