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Evaluation of animal-free systems in human deatal pulp cells using a spinal cord injury model

Research Project

Project/Area Number 26463001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionGifu University

Principal Investigator

Kawaguchi Tomoko  岐阜大学, 医学部附属病院, 医員 (30509815)

Co-Investigator(Kenkyū-buntansha) 飯田 一規  岐阜大学, 大学院医学系研究科, 助教 (30585237)
玉置 也剛  岐阜大学, 大学院医学系研究科, 助教 (40585303)
柴田 敏之  岐阜大学, 大学院医学系研究科, 教授 (50226172)
畠山 大二郎  岐阜大学, 医学部附属病院, 講師 (60377653)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords再生歯学 / 歯髄由来細胞 / iPS細胞
Outline of Final Research Achievements

Human dental pulp cells (hDPC) are a promising resource for regenerative medicine and tissue engineering. However, current protocols use reagents of animal origin (mainly fetal bovine serum) that carry the potential risk of infectious diseases and unwanted immunogenicity. Here, we report a chemically defined protocol to isolate and maintain the growth and differentiation potential of hDPCs. The chemically defined culture conditions can be used for the safe establishment of iPSCs and will find utility in applications for cell-based regenerative medicine.
The central nervous system in adult mammals cannot be repaired spontaneously after spinal cord injury (SCI). However, SCI treatment has improved in recent years following the development of cell transplantation therapy. We recently found that basic fibroblast growth factor (FGF2)-pre-treated hDPCs cultured under chemically defined conditions can improve recovery in rat SCI models.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (9 results)

  • [Journal Article] Derivation of iPSCs after culture of human dental pulp cells under defined conditions2014

    • Author(s)
      Takeda-Kawaguchi T, Sugiyama K, Chikusa S, Iida K, Aoki H, Tamaoki N, Hatakeyama D, Kunisada T, Shibata T, Fusaki N, Tezuka K.
    • Journal Title

      PLoS One. 2014 Dec 18;9(12):e115392

      Volume: 18 Issue: 12 Pages: 1-15

    • DOI

      10.1371/journal.pone.0115392

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The homeobox gene DLX4 promotes generation of human induced pluripotent stem cells2014

    • Author(s)
      Naritaka Tamaoki, Kazutoshi Takahashi, Hitomi Aoki, Kazuki Iida, Tomoko Kawaguchi, Daijirou Hatakeyama, Masatoshi Inden, Naoyuki Chosa, Akira Ishisaki, Takahiro Kunisada, Toshiyuki Shibata, Naoki Goshima, Shinya Yamanaka, and Ken-ichi Tezuka
    • Journal Title

      Scientific Reports

      Volume: なし Issue: 1 Pages: 7283-7283

    • DOI

      10.1038/srep07283

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 脊髄損傷モデルラットにおけるFGF2処理ヒト歯髄細胞移植の回復促進効果:細胞・FGF2単独投与との比較2017

    • Author(s)
      杉山健、飯田一規、川口 知子、畠山大二郎、柴田敏之、福光秀文、手塚建一
    • Organizer
      第16回日本再生医療学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] FGF2処理ヒト歯髄細胞移植の回復促進効果:細胞・FGF2単独投与との比較2016

    • Author(s)
      杉山健、川口 知子、手塚建一
    • Organizer
      第17回運動器科学研究会
    • Place of Presentation
      大阪大学中之島センター
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脊髄損傷治療を目的としたヒト歯髄細胞のFGF2に対する個人差の検討2016

    • Author(s)
      杉山健、飯田一規、川口 知子、畠山大二郎、柴田敏之
    • Organizer
      第70回日本口腔科学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-04-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脊髄損傷治療を目的としたヒト歯髄細胞のFGF2に対するドナー個人差の検討2016

    • Author(s)
      杉山健、飯田一規、玉置也剛、川口 知子、畠山大二郎、柴田敏之、手塚建一
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪 大阪国際会議場
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 脊髄損傷治療を目的としたヒト歯髄細胞のbFGFに対する応答性のお検討2015

    • Author(s)
      杉山健、飯田一規、玉置也剛、川口 知子、畠山大二郎、柴田敏之
    • Organizer
      第60回日本口腔外科学会総会・学術大会
    • Place of Presentation
      名古屋 名古屋国際会議場
    • Year and Date
      2015-10-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 脊髄損傷治療を目的としたヒト歯髄細胞のbFGFに対する応答性のお検討2015

    • Author(s)
      杉山健、川口 知子、手塚 建一
    • Organizer
      第16回運動器科学研究会
    • Place of Presentation
      鹿児島 南日本新聞会館
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 脊髄損傷治療を目的としたヒト歯髄細胞のbFGFに対する応答性のお検討2015

    • Author(s)
      杉山健、川口 知子、手塚 建一
    • Organizer
      第33回日本骨代謝学会学術集会
    • Place of Presentation
      東京 京王ブラザホテル
    • Year and Date
      2015-07-23
    • Related Report
      2015 Research-status Report
  • [Presentation] 脊髄損傷ラットを用いたヒト歯髄細胞移植におけるドナー個人差の検討2014

    • Author(s)
      杉山 健、飯田 一規、玉置 也剛、川口 知子、畠山 大二郎、柴田 敏之
    • Organizer
      第59回日本口腔外科学会総会
    • Place of Presentation
      千葉 幕張メッセ 国際会議場
    • Year and Date
      2014-10-17 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Zinc Finger Nucleaseを用いたヒト歯髄細胞におけるHLA-A2の遺伝子改変2014

    • Author(s)
      千種 俊士、川口 知子、手塚 建一
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪 国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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