Analysis of metastasis and Wnt signaling in oral cancer using cervical lymph node metastasis model

• Project/Area Number: 26463002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Osaka University
• Principal Investigator: Iwai Soichi 大阪大学, 歯学部附属病院, 講師 (10362675)
• Co-Investigator(Renkei-kenkyūsha): YURA Yoshiaki 大阪大学, 歯学研究科, 教授 (00136277) ; NIKI Atsuko (YONEKAWA Atsuko) 大阪大学, 歯学部附属病院, 医員 (70600914)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 癌の浸潤・転移 / シグナル伝達 / Wnt シグナル / 口腔扁平上皮癌 / 浸潤能 / 高転移能株 / ウイントシグナル / 口腔扁平上皮癌細胞 / 転移能

Outline of Final Research Achievements

It is very important for cancer therapy to analysis of tumor invasion and metasis.
We established highly lymph nodes metastatic cell line from oral squamous cell carcinoma (OSCC) cells after in vivo selection, and investigated the involvement of Wnt signaling in metastasis using those OSCC cells with different metastatic potential. We demonstrated that Wnt5b is involved in cell motility through the activation of Cdc42 and RhoA via the non-canonical Wnt signaling pathway, and the elevated expression of Wnt5b may become an important index for the evaluation of OSCC invasion and metastasis. Furthermore, Wnt5b may become a promising therapeutic target for the prevention of OSCC metastasis

Research Products

• [Presentation] 新しい3次元組織体による口腔扁平上皮癌の遊走能・浸潤能及びリンパ管浸潤能の解析 (2016)
  • Author(s): 岩井聡一、岸本聡子、竹下彰範、濱田正和、西山今日子、今井智章、加藤逸郎、中澤光博
  • Organizer: 第61回日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ 千葉県 千葉市
  • Year and Date: 2016-11-24
• [Presentation] Wnt5b promotes the cell invasion and migration essential to the metastasis of oral squamous cell carcinoma cell through activation of Cdc42 and RhoA (2015)
  • Author(s): Soichi Iwai, Akinori Takeshita, Satoko Kishimoto, Yoshihiro Morita, Atsuko Niki-Yonekawa, Masakazu Hamada, Yoshiaki Yura
  • Organizer: European Cancer Congress (ECC) 2015
  • Place of Presentation: Wien Austria
  • Year and Date: 2015-09-25
  • Int'l Joint Research
• [Presentation] ヒト口腔扁平上皮癌におけるWntと上皮間葉転換の関連についての検討 (2015)
  • Author(s): 岸本聡子、岩井聡一、森田祥弘、竹下彰範、仁木敦子、多田晋也、由良義明
  • Organizer: 第69回日本口腔科学会総会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-05-13 – 2015-05-15
• [Presentation] ヒト口腔扁平上皮癌におけるWntと上皮間葉転換の関連についての検討 (2015)
  • Author(s): 岸本聡子、岩井聡一、森田祥弘、竹下彰範、仁木敦子、多田晋也、由良義明
  • Organizer: 第69回日本口腔科学会学術集会
  • Place of Presentation: 大阪国際会議場 （大阪府大阪市）
  • Year and Date: 2015-05-13