Functional analysis of GSK3beta in human oral cancer stem cells

• Project/Area Number: 26463005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Shigeishi Hideo 広島大学, 医歯薬保健学研究院(歯), 助教 (90397943)
• Co-Investigator(Kenkyū-buntansha): 太田 耕司 広島大学, 病院(歯), 助教 (20335681) ; 小野 重弘 広島大学, 医歯薬保健学研究院(歯), 助教 (70379882) ; 東川 晃一郎 広島大学, 病院(歯), 講師 (80363084)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 口腔癌 / 癌幹細胞 / ALDH1 / GSK3β / ＡＬＤＨ１

Outline of Final Research Achievements

CD44 and aldehyde dehydrogenase 1 (ALDH1) has been proved useful marker for the identification of cancer stem cells (CSCs). We previously reported that glycogen synthase kinase 3(GSK3beta) is involved in the regulation of self-renewal ability of oral squamous cell carcinoma (OSCC) CSC (Shigeishi et al. 2013). We demonstrate that GSK3beta play a major role in the maintenance of stemness of CD44high/ALDH1high HNSCC cells. Additionally, our results indicate a close relationship between CSC and mesenchymal character in OSCC.

Research Products

• [Journal Article] CD44(high) /ALDH1(high) head and neck squamous cell carcinoma cells exhibit mesenchymal characteristics and GSK3β-dependent cancer stem cell properties. (2016)
  • Author(s): Seino S, Shigeishi H, Hashikata M, Higashikawa K, Tobiume K, Uetsuki R, Ishida Y, Sasaki K, Naruse T, Rahman MZ, Ono S, Simasue H, Ohta K, Sugiyama M, Takechi M.
  • Journal Title: J Oral Pathol Med Volume: 45 Issue: 3 Pages: 180-188
  • DOI: 10.1111/jop.12348
  • Peer Reviewed / Open Access
• [Journal Article] Snail-induced CD44high cells in HNSCC with high ABC transporter capacity exhibit potent resistance to cisplatin and docetaxel. (2016)
  • Author(s): Hashikata M, Shigeishi H, Okui G, Yamamoto K, Tobiume K, Seino S, Uetsuki R, Kato H, Ishioka Y, Ono S, Ohta K, Higashikawa K, Sugiyama M, Takechi M.
  • Journal Title: Int J Clin Exp Pathol Volume: 9(8) Pages: 7908-7918
  • Peer Reviewed / Open Access
• [Journal Article] Elevation in 5-FU-induced apoptosis in Head and Neck Cancer Stem Cells by a combination of CDHP and GSK3β inhibitors (2015)
  • Author(s): Shigeishi H, Biddle A, Gammon L, Rodini CO, Yamasaki M, Seino S, Sugiyama M, Takechi M, Mackenzie IC.
  • Journal Title: J Oral Pathol Med Volume: 44(3) Issue: 3 Pages: 201-207
  • DOI: 10.1111/jop.12230
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Risk factors for postoperative complications following oral surgery. (2015)
  • Author(s): Shigeishi H, Ohta K, Takechi M.
  • Journal Title: Journal of Applied Oral Science Volume: 23(4) Issue: 4 Pages: 419-423
  • DOI: 10.1590/1678-775720150130
  • Peer Reviewed / Open Access
• [Journal Article] Clinicopathological analysis of salivary gland carcinomas and literature review. (2015)
  • Author(s): Shigeishi H, Ohta K, Okui G, Seino S, Hashikata M, Yamamoto K, Ishida Y, Sasaki K, Naruse T, Rahman MZ, Uetsuki R, Nimiya A, Ono S, Shimasue H, Higashikawa K, Sugiyama M, Takechi M.
  • Journal Title: Mol Clin Oncol Volume: 3(1) Pages: 202-206
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] CD44highESAlow 口腔扁平上皮癌細胞における抗癌剤抵抗性とそのメカニズムの解析 (2015)
  • Author(s): 箸方 美帆, 重石 英生, 清野 紗矢香, 太田 耕司, 東川 晃一郎，武知 正晃．
  • Organizer: 第25回日本口腔内科学会学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2015-09-18
• [Presentation] CD44high/ALDH1high口腔扁平上皮癌細胞における癌幹細胞形質の解析 (2015)
  • Author(s): 清野 紗矢香, 重石 英生, 奥井 岳, 箸方 美帆, 植月 亮, 山本 一博, 小野 重弘, 東川 晃一郎, 太田 耕司, 島末 洋, 武知 正晃．
  • Organizer: 日本組織培養学会第88回大会
  • Place of Presentation: 広島
  • Year and Date: 2015-05-26
• [Presentation] 口腔扁平上皮癌細胞における5-FU抵抗性について (2014)
  • Author(s): 箸方美帆，重石英生，奥井岳，山本一博，清野紗矢香，植月亮，東川晃一郎，武知正晃
  • Organizer: 第51回 日本口腔組織培養学会学術大会・総会
  • Place of Presentation: 北九州市、九州歯科大学
  • Year and Date: 2014-11-15
• [Presentation] 口腔扁平上皮癌におけるALDH１発現と臨床病理学的指標との関わり (2014)
  • Author(s): 清野紗矢香，重石英生，奥井 岳，箸方美帆，植月 亮，山本一博，小野重弘，東川晃一郎，武知正晃
  • Organizer: 第62回日本口腔科学会 中国・四国地方部会
  • Place of Presentation: 徳島市,徳島大学歯学部
  • Year and Date: 2014-10-25