The study of TMEM135 function in synaptic vesicles

• Project/Area Number: 26463066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: Higuchi Hitoshi 岡山大学, 大学病院, 講師 (30423320)
• Co-Investigator(Renkei-kenkyūsha): SOGAWA Chiharu 岡山大学, 大学院医歯薬学総合研究科, 講師 (10253022)
• Research Collaborator: IKEDA Akihiro University of Wisconsin-Madison, Department of Medical Genetics, Professor ; TAJIRI Ayako 岡山大学, 大学院医歯薬学総合研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Transmembrane135 / 中枢細胞 / シナプス小胞 / TMEM135 / 中枢神経 / 老化 / 神経変性 / シナプス / 神経

Outline of Final Research Achievements

The gene decoding projects in the world identified all genome sequence of human, mouse et al., and they indicated that there were still a lot of unknown genes and proteins. In this study, I evaluated the function of Tmem135(transmenbrane135) that was one of unknown gene, in synaptic vesicles. In results, TMEM135 was expressed in hippocampus neurons, and it was suggested the possibility that TMEM135 was contributed to the glutamate uptake of the synaptic vesicles.

Research Products

• [Int'l Joint Research] ウィスコンシン州立大学マディソン校(米国)
• [Int'l Joint Research] University of Wisconsin-Madison/Department of Medical Genetics(米国)
• [Journal Article] Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies (2016)
  • Author(s): Wei-Hua Lee, Hitoshi Higuchi, Sakae Ikeda, Erica L Macke, Tetsuya Takimoto, Bikash R Pattnaik, Che Liu, Li-Fang Chu, Sandra M Siepka, Kathleen J Krentz, C Dustin Rubinstein, Robert F Kalejta, James A Thomson, Robert F Mullins, Joseph S Takahashi, Lawrence H Pinto, Akihiro Ikeda
  • Journal Title: eLife Volume: 5
  • DOI: 10.7554/elife.19264
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic basis of agedependent synaptic abnormalities in the retina (2015)
  • Author(s): Hitoshi H, Macke EL, Lee WH, Miler SA, Xu JC, Ikeda S, Ikeda A
  • Journal Title: Mamm Genome Volume: 26 Issue: 1-2 Pages: 21-32
  • DOI: 10.1007/s00335-014-9546-7
  • Peer Reviewed
• [Presentation] The role of Tmem135 in retinal aging and mitochondrial dynamics (2015)
  • Author(s): Wei-Hua Lee, Hitoshi Higuchi, Erica Macke,, Che Liu , Li-Fang Chu , John B. Troy , Robert F. Mullins, Joseph Takahashi , Lawrence Pinto , Akihiro Ikeda
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology 2015
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 2015-05-03 – 2015-05-07
• [Presentation] The role of Tmem135 in retinal aging and mitochondrial dynamics (2015)
  • Author(s): Wei-Hua Lee, Hitoshi Higuchi, Erica Macke, Che Liu, Li- Fang Chu, John B. Troy, Robert F. Mullins, Joseph Takahashi, Lawrence Pinto, Akihiro Ikeda
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 2015-05-03
  • Int'l Joint Research