The role of ubiquitin proteasome system in cardioprotection by volatile anesthetics
Project/Area Number |
26463081
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Osaka Kyoiku University (2015-2017) Osaka Dental University (2014) |
Principal Investigator |
Miyamae Masami 大阪教育大学, 保健センター, 教授 (20298821)
|
Co-Investigator(Kenkyū-buntansha) |
竹村 元三 朝日大学, 歯学部, 教授 (40283311)
金田 一弘 大阪歯科大学, 歯学部, 講師(非常勤) (90533886)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | sevoflurane / ischemia-reperfusion / heart / ubiquitin-proteasome / guine pig / ubiquitin proteasome / guinea pig / autophagy / ubiquitin / proteasome / sevofllurane / ubiqitin system / volatile anesthetics / ischemia / reperfusion |
Outline of Final Research Achievements |
Administration of sevoflurane prior to ischemia-reperfusion confers cardioprotective effects (sevoflurane preconditioning). We examined the role of ubiquitin proteasome system in this protective effects. Isolated guinea pigs hearts were subjected to 30 min ischemia, followed by 120 min reperfusion (CTL). Anesthetic preconditioning was elicited with 2% sevoflurane for ten minutes prior to ischemia (SEVO). Infarct size was reduced in SEVO compared with CTL. Immunohistochemical analysis revealed that there were less myoglobin-negative cells and ubiquitin and LC 3 stain were increased in SEVO. Electron microscopic study showed that there were more abundant autophagosomes in SEVO. These results suggests that ubiquitin proteasome system plays a role in cardioprotective effects of sevoflurane associated with autophagy.
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Report
(5 results)
Research Products
(21 results)
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[Journal Article] Three dimensional ultrastructure of capillary endothelial glycocalyx under normal and septic conditions.2017
Author(s)
Okada H, Takemura G, Suzuki K, Oda K, Takada C, Hotta Y, Miyazaki N, Tsujimoto A, Muraki I, Ando Y, Zaikokuji R, Matsumoto A, Kitagaki H, Tamaoki Y, Usui T, Doi T, Yoshida T, Yoshida S, Ushikoshi H, Toyoda I, Ogura S.
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Journal Title
Critical Care.
Volume: 26
Issue: 1
Pages: 261-261
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Non-invasive tissue characterization of lung tumors using integrated backscatter intravascular ultrasound: An ex vivo comparative study with pathological diagnosis.2016
Author(s)
Ito F, Kawasaki M, Ohno Y, Toyoshi S, Morishita M, Kaito D, Yanase K, Funaguchi N, Asano M, Endo J, Mori H, Kobayashi K, Nishigaki K, Miyazaki T, Takemura G, Minatoguchi S.
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Journal Title
Chest.
Volume: 149
Issue: 5
Pages: 1276-1284
DOI
Related Report
Peer Reviewed
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[Journal Article] An oxidative stress biomarker, urinary 8-hydroxy-2'-deoxyguanosine, predicts cardiovascular-related death after steroid therapy for patients with active cardiac sarcoidosis.2016
Author(s)
Myoren T, Kobayashi S, Oda S, Nanno T, Ishiguchi H, Murakami W, Okuda S, Okada M, Takemura G, Suga K, Matsuzaki M, Yano M.
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Journal Title
Int J Cardiol
Volume: 17
Pages: 206-213
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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