Central nervous regeneration by transplantation of dedifferentiated fat cell in mouse brain infarction model

• Project/Area Number: 26463082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KOTANI Junichiro 大阪歯科大学, 歯学部, 名誉教授 (40109327)
• Co-Investigator(Kenkyū-buntansha): 岸本 直隆 大阪歯科大学, 歯学部, 講師 (50610911) ; 百田 義弘 大阪歯科大学, 歯学部, 教授 (60247880)
• Co-Investigator(Renkei-kenkyūsha): MATSUYAMA Tomohiro 兵庫医科大学, 医学部, 教授 (10219529) ; MAEDA Mitsuyo 公益財団法人先端医療振興財団, 再生医科学研究所, 研究員 (40122080)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脱分化脂肪細胞 / 脳梗塞 / 中枢神経 / 細胞移植 / 再生 / 幹細胞 / 組織工学 / 再生医療 / 中枢神経再生

Outline of Final Research Achievements

We isolated the dedifferentiated fat (DFAT) by ceiling culture of mature adipocytes obtained from human buccal fat pads.DFAT expressed neuronal markers nestin, sox2. Moreover, it had properties similar to those of mesenchymal stem cells from Flow cytometric analysis. Although there was no difference in the infarct side surface area by xenografting these cells to a cerebral infarction disease model mouse, functional recovery was observed in the behavioral test. In addition, DFAT transplanted into mice was detected in cerebral infarct lesion. Therefore, the possibility that transplanted DFAT influences brain after infarction and contributes to promotion of functional recovery was considered.
This finding suggested the possibility of a new source of cell replacement therapy for the treatment of central nervous disorders.

Research Products

• [Presentation] 脱分化脂肪細胞の神経系分化とマウス脳梗塞モデルへの細胞移植による脳分布 (2016)
  • Author(s): 覚道 知樹、岸本 直隆、土居 亜紀子、中込 隆之、百田 義弘、松山 知弘
  • Organizer: 第59回日本脳循環代謝学会学術集会
  • Place of Presentation: 徳島市 あわぎんホール
  • Year and Date: 2016-11-11
• [Presentation] 脱分化脂肪細胞による中枢神経再生 (2016)
  • Author(s): 覚道 知樹、岸本 直隆、百田 義弘
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡市 国際会議場/福岡サンパレス
  • Year and Date: 2016-10-22
• [Presentation] 脱分化脂肪細胞の神経系細胞分化 (2015)
  • Author(s): 覚道知樹、岸本直隆、百田義弘
  • Organizer: 第43回日本歯科麻酔学会総会・学術集会
  • Place of Presentation: 学術総合センター（東京都）
  • Year and Date: 2015-10-31
• [Presentation] The possibility of dedifferentiated fat cells for tissue engineering (2015)
  • Author(s): 岸本直隆
  • Organizer: McGill University, Faculty of Dentistry Research Seminar
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2015-09-18
  • Int'l Joint Research