| Project/Area Number |
26463092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
Yuto Takeuchi 大阪大学, 歯学部附属病院, 医員 (60721454)
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| Co-Investigator(Kenkyū-buntansha) |
三原 聖美 大阪大学, 歯学研究科, 招へい教員 (00551920)
山城 隆 大阪大学, 歯学研究科, 教授 (70294428)
柳田 剛志 岡山大学, 大学病院, 講師 (90534793)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 象牙芽細胞 / エピジェネティクス / 象牙質 / エピジェネティック / 分化 / クロマチン修飾 / 歯髄幹細胞 / 再生医療 |
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| Outline of Final Research Achievements |
Dentin is similar to bone in terms of its matrix protein composition and abundance of molecular cascades participating in odontoblast differentiation that are shared with osteoblast differentiation. On the other hand, dentin does not undergo remodeling, and odontoblasts secrete dentin throughout their lifespan. In this study, we topically applied LiCl, an activator of canonical Wnt signaling, as a novel capping material in order to activate reparative dentin formation. MicroCT and microscopic analyses demonstrated that the topical application of LiCl induced dentin repair, including the formation of a complete dentin bridge. In contrast, a dentin bridge was not induced in the control group treated with pulp capping with material carriers alone. These results provide a scientific basis for the biomimetic regeneration of dentin using LiCl to activate dentine regeneration presumably through epigenetic modulation .
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