Elucidation of epigenetic regulation in regeneration of tongue muscle

• Project/Area Number: 26463103
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: Fukui Tadayoshi 鶴見大学, 歯学部, 非常勤講師 (10267544)
• Co-Investigator(Kenkyū-buntansha): 安藤 準 鶴見大学, 歯学部, 講師 (00282765) ; 大久保 力廣 鶴見大学, 歯学部, 教授 (10223760) ; 成山 明具美 鶴見大学, 歯学部, 助教 (90440304)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エピジェネティクス / 舌 / 発生 / 舌筋 / 再生 / iPS

Outline of Final Research Achievements

This study aims at establishment of condition to induce tongue muscle from non-muscle cells especially thorough epigenetic approaches. We tried to induce tongue muscle cells from iPS cells. However, we could not find efficient condition for the induction of tongue muscle. We then investigated epigenetic regulation of embryonic tongue formation in vivo. We found that expression of a histone H3K9 methyltransferase gene, G9a, is required for proper tongue formation.

Research Products

• [Journal Article] <i>MyoD</i> is regulated by the miR-29a-<i>Tet1</i> pathway in C2C12 myoblast cells (2016)
  • Author(s): Chikenji A, Ando H, Nariyama M, Suga T, Iida R, Gomi K.
  • Journal Title: Journal of Oral Science Volume: 58 Issue: 2 Pages: 219-229
  • DOI: 10.2334/josnusd.15-0684
  • NAID: 130005158882
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed
• [Journal Article] Bone augmentation around a dental implant using demineralized bone sheet containing biologically active substances (2016)
  • Author(s): Shirai M, Yamamoto R, Chiba T, Komatsu K, Shimoda S, Yamakoshi Y, Oida S, Ohkubo C.
  • Journal Title: Dental Materials Journal Volume: 35 Issue: 3 Pages: 470-478
  • DOI: 10.4012/dmj.2016-026
  • NAID: 130005155180
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access
• [Journal Article] Digital Relief of the Mental Foramen for a CAD/CAM-Fabricated Mandibular Denture. (2016)
  • Author(s): Ohkubo C, Park EJ, Kim TH, Kurtz KS.
  • Journal Title: J Prosthodont. Volume: - Issue: 2 Pages: 189-192
  • DOI: 10.1111/jopr.12476
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of a multilayered DNA/protamine coating on titanium implants on bone responses (2016)
  • Author(s): Sakurai T, Yoshinari M2, Toyama T3, Hayakawa T4, Ohkubo C
  • Journal Title: J Biomed Mater Res Part A Volume: 104A Issue: 6 Pages: 1500-1509
  • DOI: 10.1002/jbm.a.35679
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Role of phosphodiesterase 4 expression in the epac1 signaling-dependent skeletal muscle hypertrophic action of clenbuterol． (2016)
  • Author(s): Ohnuki Y，Umeki D，Mototani Y，Shiozawa K，Nariyama M，Ito A，Kawamura N，Yagisawa Y，Jin H，Cai W，Suita K，Saeki Y，Fujita T，Ishikawa Y，Okumura S.
  • Journal Title: Physiological reports. Volume: 4 Issue: 10 Pages: e12791-e12791
  • DOI: 10.14814/phy2.12791
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative measurements of the articular areas of mandibular condyles with temporomandibular joint disorders using magnetic resonance imaging. (2016)
  • Author(s): Kohi K, Igarashi C, Sugisaki M, Osano T, Sato Y, Kobayashi K, Ohkubo C.
  • Journal Title: Cranio. Volume: 34(2) Issue: 2 Pages: 79-87
  • DOI: 10.1179/2151090314y.0000000038
  • Peer Reviewed
• [Journal Article] Occlusal contact of fixed implant prostheses using functional bite impression technique. (2015)
  • Author(s): Suzuki Y, Shimpo H, Ohkubo C.
  • Journal Title: Implant Dent. Volume: 24(1) Pages: 42-46
  • DOI: 10.1097/id.0000000000000175
  • Peer Reviewed
• [Journal Article] Fabrication of crown restoration retrofitting to existing clasps using CAD/CAM: Fitness accuracy and retentive force (2015)
  • Author(s): Ozawa D, Suzuki Y, Kawamura N, Ohkubo C
  • Journal Title: Journal of Prosthodontic Research Volume: 59 Issue: 2 Pages: 136-143
  • DOI: 10.1016/j.jpor.2015.01.002
  • NAID: 130007936693
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed
• [Journal Article] Influence of Occlusal Force on Electroencephalograms in Edentulous Patients. (2015)
  • Author(s): Morokuma M, Yoneyama Y, Matsuda R, Hosoi T, Ohkubo C.
  • Journal Title: J Prosthodont Volume: in press Issue: 7 Pages: 532-537
  • DOI: 10.1111/jopr.12263
  • Peer Reviewed
• [Journal Article] Fixation of magnet assembly to denture base using alternative resins (2015)
  • Author(s): Okayama S, Suzuki Y, Shimpo H, Ohkubo C.
  • Journal Title: Dental Materials Journal Volume: 34 Issue: 3 Pages: 364-370
  • DOI: 10.4012/dmj.2014-348
  • NAID: 130005074660
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access
• [Journal Article] Functions of miR-1 and miR-133a during the postnatal development of masseter and gastrocnemius muscles. (2015)
  • Author(s): Nariyama M, Mori M, Shimazaki E, Ando H, Ohnuki Y, Abo T, Yamane A, Asada Y
  • Journal Title: Mol Cell Biochem Volume: 404 Issue: 1-2 Pages: 17-27
  • DOI: 10.1007/s11010-015-2450-y
  • Peer Reviewed
• [Journal Article] Clinical application of removable partial dentures using thermoplastic resin&mdash;Part I: Definition and indication of non-metal clasp dentures (2014)
  • Author(s): Fueki K, Ohkubo C, Yatabe M, Arakawa I, Arita M, Ino S, Kanamori T, Kawai Y, Kawara M, Komiyama O, Suzuki T, Nagata K, Hosoki M, Masumi S, Yamauchi M, Aita H, Ono T, Kondo H, Tamaki K, Matsuka Y, Tsukasaki H, Fujisawa M, Baba K, Koyano K,Yatani H.
  • Journal Title: Journal of Prosthodontic Research Volume: 58 Issue: 1 Pages: 3-10
  • DOI: 10.1016/j.jpor.2013.12.002
  • NAID: 130004953481
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed / Open Access
• [Journal Article] An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models. (2014)
  • Author(s): Urushibara Y, Ohshima T, Sato M, Hayashi Y, Hayakawa T, Maeda N, Ohkubo C.
  • Journal Title: Dent Mater J Volume: 33 Pages: 402-414
  • NAID: 130004519328
  • Peer Reviewed / Open Access
• [Journal Article] Pressure distribution of implant-supported removable partial dentures with stress-breaking attachments (2014)
  • Author(s): Kono K, Kurihara D, Suzuki Y, Ohkubo C.
  • Journal Title: Journal of Prosthodontic Research Volume: 58 Issue: 2 Pages: 115-120
  • DOI: 10.1016/j.jpor.2014.01.002
  • NAID: 130004953496
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed
• [Journal Article] Clinical application of removable partial dentures using thermoplastic resin. Part II: Material properties and clinical features of non-metal clasp dentures (2014)
  • Author(s): Fueki K, Ohkubo C, Yatabe M, Arakawa I, Arita M, Ino S, Kanamori T, Kawai Y, Kawara M, Komiyama O, Suzuki T, Nagata K, Hosoki M, Masumi S, Yamauchi M, Aita H, Ono T, Kondo H, Tamaki K, Matsuka Y, Tsukasaki H, Fujisawa M, Baba K, Koyano K,Yatani H.
  • Journal Title: Journal of Prosthodontic Research Volume: 58 Issue: 2 Pages: 71-84
  • DOI: 10.1016/j.jpor.2014.03.002
  • NAID: 130004953491
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed / Open Access
• [Journal Article] The influence of vertical dimension of occlusion changes on the electroencephalograms of complete denture wearers (2014)
  • Author(s): Matsuda R, Yoneyama Y, Morokuma M, Ohkubo C.
  • Journal Title: Journal of Prosthodontic Research Volume: 58 Issue: 2 Pages: 121-126
  • DOI: 10.1016/j.jpor.2014.01.003
  • NAID: 130004953497
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed
• [Journal Article] Effects of in vitro cyclic dislodging on retentive force and removal torque of three overdenture attachment systems. (2014)
  • Author(s): Kobayashi M, Srinivasan M, Ammann P, Perriard J, Ohkubo C, Muller F, Belser UC, Schimmel M.
  • Journal Title: Clin Oral Implants Res Volume: 25 Issue: 4 Pages: 426-434
  • DOI: 10.1111/clr.12156
  • Peer Reviewed
• [Journal Article] Bone responses to zirconia implants with a thin carbonate-containing hydroxyapatite coating using a molecular precursor method (2014)
  • Author(s): Hirota M, Hayakawa T, Ohkubo C, Sato M, Hara H, Toyama T, Tanaka Y.
  • Journal Title: J Biomed Mater Res B Appl Biomater. Volume: 102 Issue: 6 Pages: 1277-1288
  • DOI: 10.1002/jbm.b.33112
  • Peer Reviewed
• [Journal Article] In vitro assessment of mandibular single/two implant-retained overdentures using stress-breaking attachments. (2014)
  • Author(s): Kono K, Kurihara D, Suzuki Y, Ohkubo C
  • Journal Title: Implant Dent Volume: 23 Pages: 456-62
  • DOI: 10.1097/id.0000000000000099
  • Peer Reviewed
• [Presentation] 胎生期マウスの舌横紋筋細胞分化におけるmiR-1の役割 (2014)
  • Author(s): 福井只美、幕内俊介、片山理奈、山根明
  • Organizer: 第73回日本矯正歯科学会大会
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2014-10-20 – 2014-10-22
• [Presentation] 鶴見大学歯学部小児歯科学講座における学生実習の現状 (2014)
  • Author(s): 成山明具美, 船山ひろみ, 守安克也, 井出正道, 朝田芳信
  • Organizer: 第28回日本小児歯科学会関東地方会大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県大宮市）
  • Year and Date: 2014-09-28
• [Presentation] 咬筋における miR-206 とアセチルコリン受容体形成因子との関連性について (2014)
  • Author(s): 島崎絵美、成山明具美、安藤 準、山根 明、朝田芳信
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-27
• [Presentation] 咬合様式の違いが咬筋におけるmiR-206の発現に及ぼす影響 (2014)
  • Author(s): 島崎絵美、森 愛美、成山明具美、山根 明、朝田芳信
  • Organizer: 第52回日本小児歯科学会大会
  • Place of Presentation: 品川区立総合区民会館（東京都品川区）
  • Year and Date: 2014-05-16