Epigenetics in the postnatal development of masticatory muscles

• Project/Area Number: 26463127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: Nariyama Megumi 鶴見大学, 歯学部, 助教 (90440304)
• Co-Investigator(Kenkyū-buntansha): 安藤 準 鶴見大学, 歯学部, 講師 (00282765) ; 福井 只美 鶴見大学, 歯学部, 非常勤講師 (10267544) ; 朝田 芳信 鶴見大学, 歯学部, 教授 (20184145)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 咀嚼筋 / 非咬合モデルマウス / 生後発達 / エピジェネティクス

Outline of Final Research Achievements

The regulatory pathway of miR-1 via HDAC4 and MEF2 plays a more prominent role during postnatal development in the masseter muscle than in the gastrocnemius muscle, whereas that of miR-133a via SRF plays a more prominent role in the gastrocnemius muscle than in the masseter muscle.
MiR-1 promotes the differentiation of skeletal muscle directly through the post-transcriptional inhibition of HDAC4 expression and that miR-133a promotes the proliferation of skeletal muscle directly through the post-transcriptional inhibition of SRF expression.
MiR-1 promotes the differentiation of skeletal muscle directly through the post-transcriptional inhibition of HDAC4 expression and that miR-133a promotes the proliferation of skeletal muscle directly through the post-transcriptional inhibition of SRF expression.

Research Products

• [Journal Article] Role of phosphodiesterase 4 expression in the epac1 signaling-dependent skeletal muscle hypertrophic action of clenbuterol． (2016)
  • Author(s): Ohnuki Y，Umeki D，Mototani Y，Shiozawa K，Nariyama M，Ito A，Kawamura N，Yagisawa Y，Jin H，Cai W，Suita K，Saeki Y，Fujita T，Ishikawa Y，Okumura S.
  • Journal Title: Physiological reports. Volume: 4 Issue: 10 Pages: e12791-e12791
  • DOI: 10.14814/phy2.12791
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MyoD is regulated by the miR-29a-Tet1 pathway in C2C12 (2016)
  • Author(s): Chikenji A, Ando H, Nariyama M, Suga T,Iida R, Gomi K
  • Journal Title: Journal of Oral Science Volume: 58
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functions of miR-1 and miR-133a during the postnatal development of masseter and gastrocnemius muscles. (2015)
  • Author(s): Nariyama M, Mori M, Shimazaki E, Ando H, Ohnuki Y, Abo T, Yamane A, Asada Y
  • Journal Title: Mol Cell Biochem Volume: 404 Issue: 1-2 Pages: 17-27
  • DOI: 10.1007/s11010-015-2450-y
  • Peer Reviewed
• [Presentation] Role of Microphthamia-Associated Transcription Factor in Masseter Muscle Hypertrophy Induced by β 2-adrenoceptor Stimulation (2016)
  • Author(s): Nariyama M, Ohnuki Y, Umeki D, Ito A, Kawamura N, Yagisawa Y，Okumura S, and Asada Y
  • Organizer: 第58回歯科基礎医学会
  • Place of Presentation: 札幌コンベンションセンター (北海道札幌市)
  • Year and Date: 2016-08-24
• [Presentation] Inhibitory effects of dexamethasone on bite opening-induced hypertrophy in masseter and cardiac muscles. (2016)
  • Author(s): Umeki D, Ohnuki Y, Ito A, Yagisawa Y, Nariyama M, Ishikawa M, Kawamura N Nakamura Y, and Okumura S
  • Organizer: 第58回歯科基礎医学会
  • Place of Presentation: 札幌コンベンションセンター (北海道札幌市)
  • Year and Date: 2016-08-24
• [Presentation] Epac1-dependent hypertrophic effects of β2-adrenoceptor stimulation differ between slow- and fast-twitch muscles (2016)
  • Author(s): Ohnuki Y, Umeki D, Nariyama M, Ito A, Kawamura N, Yagisawa Y, and Okumura S
  • Organizer: 第58回歯科基礎医学会
  • Place of Presentation: 札幌コンベンションセンター (北海道札幌市)
  • Year and Date: 2016-08-24
• [Presentation] Role of Microphthamia-Associated Transcription Factor in Masseter Muscle Hypertrophy Induced by β2-adrenoceptor Stimulation (2016)
  • Author(s): Nariyama M, Ohnuki Y , Umeki D, Ito A, Kawamura N, Okumura S, and Asada Y
  • Organizer: 第10回アジア小児歯科学会
  • Place of Presentation: 東京ドームホテル (東京都文京区)
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] A case of longitudinal dental management of autistic patient refused the dental treatment for several years by worse of the condition (2015)
  • Author(s): 小林和弘、濱中一将、 成山明具美 他17名
  • Organizer: 第32回日本障害者歯科学会総会および学術大会
  • Place of Presentation: 名古屋国際会議場 （愛知県名古屋市）
  • Year and Date: 2015-11-06
• [Presentation] 咬筋と腓腹筋の生後発達過程におけるmiR-1, miR-133aの機能 (2015)
  • Author(s): 成山明具美、島崎絵美、安藤 準、大貫芳樹、奥村 敏、朝田芳信
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ 新潟コンベンションセンター （新潟県新潟市）
  • Year and Date: 2015-09-11
• [Presentation] 鶴見大学歯学部小児歯科学講座における学生実習の現状 (2014)
  • Author(s): 成山明具美, 船山ひろみ, 守安克也, 井出正道, 朝田芳信
  • Organizer: 第28回日本小児歯科学会関東地方会大会
  • Place of Presentation: 大宮ソニックシティ（埼玉）
  • Year and Date: 2014-09-28
• [Presentation] 咬筋における miR-206 とアセチルコリン受容体形成因子との関連性について (2014)
  • Author(s): 島崎絵美、成山明具美、安藤 準、山根 明、朝田芳信
  • Organizer: 第56回歯科基礎医学会学術大会
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 咬合様式の違いが咬筋におけるmiR-206の発現に及ぼす影響 (2014)
  • Author(s): 島崎絵美、森 愛美、成山明具美、山根 明、朝田芳信
  • Organizer: 第52回日本小児歯科学会大会
  • Place of Presentation: 品川区立総合区民会館（東京）
  • Year and Date: 2014-05-16 – 2014-05-17
• [Book] 日本歯科評論 増刊2015 子どもの患者の治療・対応に上手くなろう！各成長ステージにおける対応ポイント (2015)
  • Author(s): 成山明具美、朝田芳信
  • Total Pages: 160
  • Publisher: ヒョーロン・パブリッシャーズ