Sphingomyelin regulates blood glucose levels by bone tissue

• Project/Area Number: 26463202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: YOSHIKAWA Yoshihiro 大阪歯科大学, 歯学部, 講師 (70434793)
• Co-Investigator(Kenkyū-buntansha): 池尾 隆 大阪歯科大学, 歯学部, 教授 (40159603) ; 岡崎 俊朗 金沢医科大学, 医学部, 教授 (40233308) ; 吉澤 達也 熊本大学, 大学院生命科学研究部(医), 准教授 (40313530)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 骨代謝 / 骨芽細胞 / スフィンゴミエリン合成酵素 / 骨芽細胞分化 / 破骨細胞分化因子 / 破骨細胞 / 糖代謝

Outline of Final Research Achievements

The skeleton has been shown recently to regulate glucose metabolism through an osteoblast-specific hormone. Here, we investigated the role of sphingomyelin in osteoblasts. Knockdown of sphingomyelin synthase 1 or 2 resulted in inhibited osteoblast differentiation and reduced osteoclast formation. We found that sphingomyelin synthase affected osteoblast function, and we suggested sphingomyelin synthase effects to regulate glucose metabolism by osteoblast.

Research Products

• [Journal Article] RNA interference-mediated knockdown of Smad1 inhibits receptor activator of nuclear factor κB ligand expression induced by BMP-2 in primary osteoblasts. (2015)
  • Author(s): Yoshikawa Y, Yoshizawa T, Domae E, Hieda Y, Takeyama A, Hirota S, Kawamoto A, Goda S, Tamura I, Kamada A, Komasa Y, Morita S, Yamagata K, Ikeo T.
  • Journal Title: Archives of Oral Biology Volume: 60 Issue: 9 Pages: 1319-26
  • DOI: 10.1016/j.archoralbio.2015.06.001
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Sphingomyelin Synthase 2 siRNA regulates osteoblast functions (2016)
  • Author(s): Yoshikawa Y, Domae E, Kamada A, Ikeo T
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（札幌市・北海道）
  • Year and Date: 2016-08-26
• [Presentation] スフィンゴミエリン合成酵素（SMS）が骨に及ぼす影響. (2015)
  • Author(s): 吉川美弘, 堂前英資, 合田征司, 田村 功, 鎌田愛子, 池尾 隆.
  • Organizer: 第13回日本再生歯科医学会学術大会・総会
  • Place of Presentation: 日本歯科大学新潟生命歯学部（新潟県・新潟市）
  • Year and Date: 2015-08-29
• [Presentation] SMS1-KOマウスにおける骨代謝異常の解析 (2014)
  • Author(s): 吉川 美弘、廣田秀逸、渡辺 研、岡崎俊朗、小正 裕、池尾 隆
  • Organizer: 第９回スフィンゴテラピィ研究会
  • Place of Presentation: 山中温泉河鹿荘ロイヤルホテル（石川県・加賀市）
  • Year and Date: 2014-07-18